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Preparation method of cabbage type rape high erucic acid material

A cabbage rape, high erucic acid technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve problems such as difficulties, and achieve the effects of reducing production costs, improving enterprise economic benefits and market competitiveness.

Inactive Publication Date: 2012-06-20
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Through traditional breeding methods, the erucic acid content can reach more than 55%, but it is very difficult to stably exceed 60%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] Select the common double-high rapeseed material with an erucic acid content of about 51 as the female parent, and use the high oleic material with an oleic acid content of about 82 as the male parent to cross, and obtain the F2 generation seeds after selfing for one generation; take 200 F2 generation seeds for half-grain 34 single grains with high erucic acid, low linoleic acid and low linolenic acid were selected for sowing; anthers were taken for microspore culture at the flowering stage, and colchicine was used as chromosome doubling to obtain double haploid (DH 106 strains of ) plants; harvest the selfing seeds on the DH plants, adopt the gas chromatograph to measure the fatty acid composition, select 22 individual plants with high erucic acid, low linoleic acid and low linolenic acid to sow, and form 22 strains; Five strains were selected for selfing, and the selfing seeds were analyzed by gas chromatography. Finally, three strains were selected, and their fatty ac...

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Abstract

The invention relates to a preparation method of a cabbage type rape high erucic acid material. FAD2 (delta 12 fatty acid desaturase) and FAD3 mutant genes are imported into a common high erucic acid material (the erucic acid content is about 50 percent) to make the synthesis of linoleic acid and linolenic acid in rapeseeds basically hindered to make the high erucic acid material synthetized intomore erucic acid; and on the other hand, since the proportion of the linoleic acid to the linolenic acid in free fatty acid is reduced and the proportion of the erucic acid rises, the site 1 and the site 3 of a glycerol skeleton can be combined with sufficient erucic acid, thereby, the erucic acid content in the rapeseeds exceeds 60 percent. The invention can provide quality raw materials for industrial erucic acid manufacturing enterprises, reduces production cost and improves enterprise economic benefits and market competitiveness.

Description

technical field [0001] The invention relates to the fields of crop genetics and breeding and agricultural application biotechnology, in particular to a method for creating high erucic acid materials of Brassica napus. Background technique [0002] High erucic acid rapeseed oil can be used as a raw material for industrial lubricants, cosmetics, surfactants, plastics, etc., and has a wide range of industrial uses. There are several domestic manufacturers specialized in processing high erucic acid rapeseed oil. Among them, the high erucic acid oil produced by Sichuan Xipu Chemical Co., Ltd. accounts for 1 / 8 of the international market and 60% of the Asian market. According to the requirements of processing enterprises, the higher the erucic acid content in rapeseed oil, the better. High erucic acid rapeseed oil can significantly reduce processing costs, improve product quality, and thus improve enterprise efficiency. [0003] Breeding high erucic acid rapeseed varieties has al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00A01H1/04
Inventor 李加纳王瑞谌利
Owner SOUTHWEST UNIV