Method for preparing gelatinous enteromorpha polysaccharide
The technology of prolifera polysaccharide and gelatinity is applied in the field of preparation of gelatinous prolifera polysaccharide, and achieves the effects of good taste, low production cost and simple process
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Embodiment 1
[0018] Take 5g of enteromorpha that has been washed, dried and crushed, passed through a 30-mesh sieve, soaked in 5 times of water for 4 hours, extracted in a water bath at 80°C for 2 hours, centrifuged, extracted with 5 times of water from the algae residue, repeated extraction 3 times, combined Extract solution; add potassium chloride to make the concentration of the extract solution reach 5%, leave it overnight to obtain a yellow-green polysaccharide gel; squeeze it through a press, remove the supernatant, add 0.5mL of 10% hydrochloric acid to the precipitate to acidify, and adjust to pH ≤2, place for 4 hours; centrifuge at 3000 rpm for 10 minutes, remove the supernatant, mix 8 mL of 10% sodium carbonate into the sediment to neutralize it to form a paste, place for 2 hours; dry and pulverize to obtain light yellow gel Enteromorpha polysaccharides.
Embodiment 2
[0020] Take 5g of Enteromorpha that has been washed, dried and crushed, passed through a 40 mesh sieve, soaked in 30 times of water for 0.5 hours, extracted in a water bath at 100°C for 0.5 hours, passed through a 200 mesh sieve, extracted with 3 times of water from the algae residue, and repeated Extract 2 times, combine the extracts; add potassium chloride to make the concentration 25%, let it stand for 2 hours to obtain a yellow-green polysaccharide gel; centrifuge at 2000 rpm for 10 minutes, remove the supernatant, add 0.5mL of 20% hydrochloric acid to acidify, Adjust to pH ≤ 2, place for 2h; centrifuge at 2000 rpm for 10min, remove the supernatant, mix in 5mL of 10% sodium hydroxide to neutralize to form a paste, place for 0.5h; dry and pulverize to obtain a light yellow gel Colloidal enteromorpha polysaccharide.
Embodiment 3
[0022] Take 5g of enteromorpha that has been washed, dried and crushed, passed through a 40-mesh sieve, soaked in 5 times of water for 0.5h, extracted in a water bath at 90°C for 1 hour, extracted with 5 times of water from the algae residue, repeated extraction twice, and combined for extraction solution, passed through a 200-mesh sieve; added magnesium chloride to make the mass concentration 5%, and left for 5 hours to obtain a yellow-green polysaccharide gel; centrifuged at 3000 rpm for 10 minutes, removed the supernatant, added 0.5mL of 10% hydrochloric acid to acidify, and adjusted to pH≤2, stand for 4h; centrifuge at 3000 rpm for 10min, remove the supernatant, mix in 10mL of 15% sodium bicarbonate to neutralize to form a paste, stand for 2h; dry and pulverize to obtain light yellow gel moss polysaccharides.
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