Method for increasing oil recovery ratio by utilizing symbiotic reproduction and complex metabolism and microbial preparation
A microbial preparation and recovery factor technology, applied in the field of CR-CM technology, can solve the problems of unstable oil production microbial communities in oil reservoirs, inability to accurately understand reservoir microorganisms, and neglect of multi-compatibility of microorganisms, so as to increase oil recovery. efficiency, prolongation of production time, and the effect of improving reservoir conditions
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[0037] Example 1 Reservoir specific oil well selection and molecular evaluation system
[0038] Reservoir survey: Investigate the geological conditions of the reservoir, including reservoir temperature, porosity, pressure, permeability, degree of recovery, remaining oil distribution, oil product characteristics, formation water salinity, etc., reflecting the macroscopic geological and physical characteristics of the reservoir The environment also includes the micro-environment of the microbial community of the oil reservoir. The present invention has no specific limitation on the microbial environment of the oil reservoir, as long as there is a microbial flora in the oil reservoir, and there is no need to specifically limit the type of microorganism. The specific applicable reservoir parameters are as follows:
[0039] Temperature3%, water content>10%, permeability>20×10 -3 μm 2 ;
[0040] 1. Extraction of metagenomic DNA from soil or water samples from oil reservoirs
[0041] The so...
Example Embodiment
[0050] Example 2 Establishment and effect evaluation of CR co-metabolism system in laboratory
[0051] In order to ensure the effect of the CR system on-site experiment, we conducted an indoor evaluation test on the CR system before the construction of each well, including emulsification and viscosity reduction test, surface tension and acidity change test. Using the Rhodococcus erythropo LY-LH-11a of the present invention, the deposit number is: CGMCC No. 3510 and Stenotrophmonas maltophilia LY-LH-11b, the deposit number is: CGMCC No. Experiments were performed on the 3509 compound bacterial agent, and the numbering system is CR-J3 system.
[0052] 1. Emulsification and viscosity reduction test
[0053] The culture medium was sterilized and put into 500mL experimental bottles, each vial was filled with 100mL, and 80g sterilized crude oil was added. Keep three blank parallel samples, and then insert two strains (Rhodococcus CGMCC No. 3510 and Stenotrophomonas CGMCC No. 3509. 5% ino...
Example Embodiment
[0068] Example 3 Activator selection and evaluation
[0069] 1. Bacteria activation experiment in reservoir formation water
[0070] The activator selected in the present invention is an emulsification activator and a gas production activator, and the formula of the emulsification activator is: 3wt‰molasses, 50wt‰C 12 Or C 16 , Dipotassium hydrogen phosphate 1wt‰, ammonium nitrate 4wt‰; the laboratory number of the emulsification activator of the present invention is CY5.
[0071] The gas production activator formula: molasses 8wt‰, bran 2wt‰, dipotassium hydrogen phosphate 1.5wt‰, and ammonium nitrate 0.5wt‰. The laboratory number of the gas generating activator of the present invention is CY9.
[0072] After the activator treatment, the total biomass was increased by 4 to 5 orders of magnitude after activation, as shown in Table 3. At the same time, the activated functional bacteria effectively inhibited harmful bacteria and achieved the purpose of selective activation.
[0073] tabl...
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