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Method for embedding vitamin A acetic ester

A vitamin, acetate technology, used in applications, food preparation, food science, etc.

Inactive Publication Date: 2010-11-17
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no domestic technology of embedding vitamin A acetate in milk fat globule membrane phospholipids

Method used

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  • Method for embedding vitamin A acetic ester
  • Method for embedding vitamin A acetic ester
  • Method for embedding vitamin A acetic ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Adopt high-performance liquid chromatography to measure the entrapment efficiency of the vitamin A acetate liposome after the embedding of gained in the present embodiment; The high-performance liquid chromatograph used is that the model that U.S. Waters Company produces is the high-performance liquid chromatograph of Waters2695, Proceed as follows:

[0020] 1. Configuration of standard solution:

[0021] Preparation of vitamin A acetate standard solutions with different concentrations: Accurately weigh an appropriate amount of vitamin A, dissolve, dilute and constant volume with methanol, and prepare 0.05μg / mL, 0.1μg / mL, 0.2μg / mL, 0.4μg / mL, 0.8μg / mL vitamin A standard solution, quantified by external standard method.

[0022] 2. Conditions for HPLC analysis:

[0023] Waters2695 high performance liquid chromatograph; Waters2487UV detector; Empower chromatographic data workstation; chromatographic column is Shiseido CAPCELL PAK C 18 Column (150mm×4.6mm×5μm); mobile ph...

Embodiment 2

[0030] 1. Mix milk fat globule membrane phospholipids, cholesterol, vitamin E and vitamin A acetate, wherein the ratio of vitamin A acetate to milk fat globule membrane phospholipids is 1:33, and the milk fat globule membrane phospholipids in the mixture are 84% and cholesterol 12.8% , vitamin E 0.7%, vitamin A acetate 2.5%; the mixture is added in dehydrated alcohol, wherein the mass volume ratio of the mixture and dehydrated alcohol is 0.1g / mL;

[0031] 2. Under the condition of 550MPa, use a rotary evaporator to evaporate the mixed solution obtained in step 1 for 20 minutes until the ethanol is completely evaporated, then add an imidazole buffer solution with a pH of 7.0, shake for 15 minutes, and obtain a hydration film;

[0032] 3. Cut the hydrated film at a high speed for 40 seconds under the conditions of a high-speed shearer with a temperature of 25°C and a rotation speed of 150r / min to obtain a suspension;

[0033] 4. The suspension is passed through a high-pressure h...

Embodiment 3

[0037] This example differs from Example 2 in that the ratio of vitamin A acetate to milk fat globule membrane phospholipids is 1:20, and the mixture contains 83% milk fat globule membrane phospholipids, 12.5% ​​cholesterol, 0.5% vitamin E, and vitamin A acetate Esters 4%.

[0038] The embedding efficiency of the method of this embodiment embedding vitamin A acetate liposome is 80%.

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PUM

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Abstract

The invention discloses a method for embedding vitamin A acetic ester, relating to the method for embedding the vitamin A acetic ester using milk fat globule membrane phospholipid. The method of the invention comprises the following steps: adding the milk fat globule membrane phospholipid, cholesterol, the vitamin A acetic ester and vitamin E to an organic solvent, removing the organic solvent through rotary evaporation, adding imidazole buffer solution, processing by a high-speed cutter to obtain crude liposome suspension, and finally processing the obtained sample by a high-pressure homogenizer. The highest embedding rate of the liposome of the vitamin A acetic ester prepared by the method reaches up to 88%, which fills the gap in the prior art.

Description

technical field [0001] The invention relates to a method for embedding vitamin A acetate, which belongs to the technical field of food engineering. Background technique [0002] Vitamin A is easily affected by light and oxygen, and is prone to oxidative degradation. There are unsaturated bonds in the vitamin A molecule, and its chemical properties are lively. It is easily oxidized in the air, or destroyed by ultraviolet radiation, and loses its physiological functions. Vitamin A, especially its free alcohol, is sensitive to oxygen, acid and ultraviolet light. Therefore effectively embedding vitamin A acetate and adding it in food has become the research content of people. The different composition of milk fat globule membrane phospholipids (especially sphingomyelin) may lead to the difference of liposome performance, which is for consumers. (Infants) Provide different nutritional functions, and also protect newborns from various bacterial and viral infections. Domesticall...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23L1/303A23L33/15
Inventor 刘宁郭晨峰
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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