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Realgar for treating melanoma

The technology of melanoma and realgar is applied in the application field in the field of skin diseases, which can solve the problem of not finding a cure for metastatic melanoma, and achieve the effect of good medicinal prospect and strong pharmacological effect.

Inactive Publication Date: 2010-11-17
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although high-dose IFN-α as adjuvant therapy can reduce the relative risk of recurrence by 25% to 30% for stage III patients, and two clinical trials have confirmed that these patients can obtain survival benefits; For patients with melanoma, the objective response rate of high-dose IL-2 can reach 15%, and the CR rate is 6.6%, and the CR patients can last for several years, but in general, there is still no treatment Most effective approach for metastatic melanoma

Method used

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  • Realgar for treating melanoma
  • Realgar for treating melanoma
  • Realgar for treating melanoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Experiment on the Apoptosis of Tumor Cells Induced by Realgar

[0035] experimental method:

[0036] 1. Determination of arsenic concentration in realgar mother liquor

[0037] 2. Determination of the half maximal inhibitory concentration (IC 50 )value

[0038] 3. Determination of aging curve

[0039] 4 Determination of growth curve

[0040] 5. Observation of cell morphology by HE staining

[0041] Drug under test: realgar

[0042] Tested cells: A375, MKN45, 8898, HepG2, L02

[0043] A375 (human melanoma cell line); MKN45 (human gastric cancer cell line); 8898 (human pancreatic cancer cell line), HepG2 (human liver cancer cell line), L02 (normal human liver cell line).

[0044] 1. Experimental materials

[0045] 1. Main instruments

[0046] Bio-Rad company microplate reader (550 type); Heraeous company carbon dioxide incubator;

[0047] Inverted Optical Microscope; Sterile Workbench

[0048] 96, 24, 6-well cell culture plates; magnetic stirrer

[0049] 2. Ma...

Embodiment 2

[0113] Experiment on the Apoptosis of Tumor Cells Induced by Realgar (2)

[0114] experimental method:

[0115] 1. Flow Cytometry

[0116] 2. Determination of the content of LDH and AKP in the cell supernatant

[0117] 3. Determination of the content of total LDH and AKP in cells

[0118] 4. Apoptotic DNA ladder detection

[0119] Drug under test: realgar

[0120] Tested cells: A375, MKN45, L02

[0121] 1. Experimental materials

[0122] 1 main instrument

[0123] Bio-Rad microplate reader (type 550); Heraeous carbon dioxide incubator; inverted optical microscope; sterile operating table; 96, 24, 6-well cell culture plates; semi-automatic biochemical analyzer

[0124] 2 main reagents

[0125] RPMI-1640 medium; propidium iodide (PI); lactate dehydrogenase kit (Beijing Beihua Kangtai Clinical Reagent Co., Ltd.); GENMED lactate dehydrogenase (LDH) total activity colorimetric quantitative detection reagent Kit (Shanghai Jiemei Gene Pharmaceutical Technology Co., Ltd.); Al...

Embodiment 3

[0197] Determination of the changes of ROS and GSH levels in cells induced by realgar

[0198] Drug under test: realgar

[0199] Tested cells: A375, HepG2, MKN45, L02

[0200] Experimental Materials:

[0201] Fluorescence microplate reader;

[0202] Thermos carbon dioxide incubator;

[0203] Inverted optical microscope;

[0204] Sterile operating table;

[0205] 96, 24, 6-well cell culture plates;

[0206] Semi-automatic biochemical analyzer

[0207] CDCFH probe (Sigma)

[0208] Glutathione Kit (Nanjing Jiancheng)

[0209] experimental method:

[0210] 1 Determination of glutathione

[0211] 1.1 Preparation of supernatant: Take 0.5ml of the sample to be tested, add 2ml of reagent-application solution, mix well, centrifuge at 3500-4000 rpm for 10 minutes, take 1ml of supernatant for color reaction.

[0212] 1.2 Color reaction:

[0213]

blank tube

standard tube

Assay tube

Reagent 1 (ml)

1.0

20μmol / LGSH standard (ml)

...

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Abstract

The invention relates to application of realgar in preparing medicaments for treating skin tumor disease melanoma. The invention finds a new medical treatment purpose of the realgar and exploits a new application field of the realgar. The realgar of the invention has good pharmacological effects, which indicates that the realgar has a good officinal prospect. The realgar does not obviously affect the cell cycle, but fulfills the aim of damaging melanoma cells by inducing cell apoptosis, so that the realgar can treat melanoma skin disease.

Description

【Technical field】 [0001] The present invention relates to the use of realgar, in particular to the application in the field of skin diseases. 【Background technique】 [0002] Melanoma is currently one of the fastest growing malignant tumors, with an annual growth rate of about 3% to 5%. Although high-dose IFN-α as adjuvant therapy can reduce the relative risk of recurrence by 25% to 30% for stage III patients, and two clinical trials have confirmed that these patients can obtain survival benefits; For patients with melanoma, the objective response rate of high-dose IL-2 can reach 15%, and the CR rate is 6.6%, and the CR patients can last for several years, but in general, there is still no treatment The most effective method for metastatic melanoma. [0003] Realgar was first recorded in "Shen Nong's Materia Medica". It is pungent and poisonous, and returns to the liver and large intestine meridians. Its component is arsenic disulfide, which has the functions of detoxificat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/36A61P35/00
Inventor 马勇陈思宇吴国华刘建文章莉陈明苍
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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