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Fermentation method of co-production of astaxanthin and Fructooligosaccharide

A technology of fructo-oligosaccharide and fermentation method, which is applied in the field of biomedicine, can solve the problems of inability to produce astaxanthin and fructo-oligosaccharide at the same time, low medium concentration, and low astaxanthin content, so as to shorten the fermentation cycle and improve the applicability Wider and lower production costs

Active Publication Date: 2010-11-17
厦门联合伟业科技有限公司
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AI Technical Summary

Problems solved by technology

[0005] At present, although there are studies involving the production of astaxanthin and fructo-oligosaccharides by using Phaffia yeast fermentation, they are all researches on the fermentation of astaxanthin or fructo-oligosaccharides alone, and the technological process includes: preparation of medium →Inoculation→Fermentation→Collection of bacteria or fermentation broth; the concentration of the medium used is low, the fermentation yield is low, and the time is long, and each batch of fermentation only produces one product, and astaxanthin and fructooligosaccharides cannot be produced at the same time. Therefore, the high cost
For example, the currently reported biomass of astaxanthin fermented by Phaffia yeast is 50-60g / L dry bacteria, the output of astaxanthin is 30-60mg / L fermentation broth, and the fermentation broth does not contain fructo-oligosaccharides; The biomass in the fermented liquid using Phaffia yeast to ferment fructo-oligosaccharides is about 10g / L, the content of astaxanthin is about 5-6mg / L, and the fermentation period is more than 48h. Although it can produce high-yield fructo-oligosaccharides (60g / L), But the content of astaxanthin is too low to be worth recycling

Method used

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  • Fermentation method of co-production of astaxanthin and Fructooligosaccharide
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  • Fermentation method of co-production of astaxanthin and Fructooligosaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0019] (1) The medium composition is: glucose 1%, yeast extract 1%, ammonium sulfate 1.5%, disodium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, calcium chloride 0.04%, water 96.16% .

[0020] (2) Calculation and weighing of cultured ingredients: Take the fermentation in a 10-ton tank as an example, and the initial fermentation volume is 4m 3 Then the initial culture of the preparation fermentation needs: glucose = 1% × 1000L / m 3 ×4m 3 ×1000g / L=40kg, yeast extract=1%×1000L / m 3 ×4m 3 ×1000g / L=40kg, (NH 4 ) 2 SO 4 =1.5%×1000L / m 3 ×4m 3 ×1000g / L=60kg, Na 2 HPO 4 =0.1%×1000L / m 3 ×4m 3 ×1000g / L=4kg, KH 2 PO 4 =0.1%×1000L / m 3 ×4m 3 ×1000g / L=4kg, MgSO 4 =0.1%×1000L / m 3 ×4m 3 ×1000g / L=4kg, CaCl 2 =0.04%×1000L / m 3 ×4m 3 ×1000g / L=1.6kg, water=96.16%×1000L / m 3 ×4m 3 ×1000g / L=3966.4kg.

[0021] (3) Preparation and sterilization of the initial medium: weigh the above ingredients and mix them evenly, add water 3.7m 3 Mix eve...

Embodiment 2

[0026] (1) The medium components are: glucose 3%, yeast extract 2.5%, ammonium sulfate 2.4%, disodium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.4%, magnesium sulfate 0.2%, calcium chloride 0.08%, water 91.02% .

[0027] (2) The weighing, preparation, sterilization, inoculation and fermentation process control of the culture medium are the same as in Example 1.

[0028] (3) Feeding: the operation of feeding is the same as in Example 1. When the feeding ends, the fermentation broth consumes 240g / L of sugar in total, the concentration of bacteria is 65g / L, and astaxanthin is 630mg / L.

Embodiment 3

[0030] (1) The medium composition is: glucose 2%, yeast extract 1.75%, ammonium sulfate 1.95%, disodium hydrogen phosphate 0.25%, potassium dihydrogen phosphate 0.25%, magnesium sulfate 0.15%, calcium chloride 0.06%, water 93.59% .

[0031] (2) The weighing, preparation, sterilization, inoculation and fermentation process control of the culture medium are the same as in Example 1.

[0032] (3) Feeding: The operation of feeding is the same as that of Example 1. When the feeding ends, the fermentation broth consumes a total of 240 g / L of sugar and the amount of bacteria is 75 g / L, and astaxanthin is 690 mg / L.

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Abstract

The invention discloses a fermentation method which uses Phaffia rhodozyma to coproduce astaxanthin and Fructooligosaccharide, comprising the following main technical flows: culturing strains, carrying out high-yield fermentation on astaxanthin, replenishing high-concentration cane sugar and glucose syrup, and controlling the condition to covert and produce Fructooligosaccharide. The invention ismainly characterized in that when astaxanthin fermentation almost ends, the mixture of high-concentration cane sugar and glucose syrup is replenished, and the Fructooligosaccharide is obtained by conversion. The method utilizes Phaffia rhodozyma cultured in one batch to simultaneously produce astaxanthin and Fructooligosaccharide at high yield, thus greatly lowering the cost for culturing Phaffiarhodozyma and producing astaxanthin.

Description

technical field [0001] The invention relates to a technology for utilizing Phaffia yeast, in particular to a co-production fermentation method for producing astaxanthin and fructooligosaccharides by using Phaffia yeast. Its technical field belongs to the field of biomedicine, and the purpose of the invention of this technology is mainly to reduce the production cost of astaxanthin and promote the popularization and application of the industrial fermentation technology of natural astaxanthin. Background technique [0002] Phaffia yeast is an important natural astaxanthin resource. The production of astaxanthin by fermentation of Phaffia yeast has the advantages of high product safety, high production efficiency, not limited by climatic conditions, small footprint, and easy to meet process conditions. It is the high-tech development direction of astaxanthin production. [0003] After long-term research by domestic scientific researchers, the fermentation production level of a...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12P19/00C12R1/645
Inventor 蔡慧农倪辉肖安风黄高凌李丽君曾琪杨远帆
Owner 厦门联合伟业科技有限公司
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