Anti-flavivirus envelope E protein monoclonal antibody and application thereof

A monoclonal antibody, flavivirus technology, applied in antiviral immunoglobulins, antiviral agents, applications, etc., can solve the problems of exacerbation of flavivirus disease, negative effects, inappropriate antibodies, etc., to achieve good broad-spectrum anti-yellow virus effect of virus
CN101891806AActive Publication Date: 2010-11-24MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
Publication Date
2010-11-24

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Abstract

The invention discloses an anti-flavivirus envelope E protein monoclonal antibody and application thereof. The anti-flavivirus envelope E protein monoclonal antibody of the invention is secreted by a mouse source hybrid tumour cell strain D2-2A10G6 with the preservation number of CGMCC No. 3292. The anti-flavivirus monoclonal antibody of the invention can be specifically combined with the function epitope of flavivirus envelope E protein. The amino acid sequence of the functional epitope of envelope E protein is SEQ ID NO: 17. The monoclonal antibody of the invention is subject to screening by indirect immunofluorescent method, and indirect enzyme-linked immunization is used for evaluating the specificity and affinity when being combined with antigen. By adopting the monoclonal antibody or immunoconjugate of the invention, flavivirus infection cell can be blocked and suckling mouse can be protected from virus attack, thus achieving the effect of inhibiting virus infection. The monoclonal antibody or immunoconjugate of the invention also can be used for flavivirus envelope E protein detection.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and more specifically, the invention discloses a functional epitope of flavivirus envelope E protein, a monoclonal antibody specifically binding to the epitope and its use in the preparation of anti-flavivirus drugs . Background technique

[0002] The genus Flavivirus (flaviviruses) of the family Flaviviridae includes more than 70 kinds of viruses, 40 of which are related to human diseases, such as Dengue virus (DENV), Japanese Encephalitis Virus (JEV), forest Encephalitis virus (Tick-borne Encephalitis Virus, TBEV), Yellow Fever Virus (Yellow Fever Virus, YFV) and West Nile Virus (West Nile Virus, WNV), etc. These viruses often cause zoonotic diseases, and most of them are mainly transmitted through arthropod vectors, which often lead to outbreaks of diseases and are one of the major public health problems in the world. More than 6 billion people are currently at risk of infection with flaviviruse...

Claims

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