Tomato pomace biological fermentation feed and preparation method thereof
A technology for biologically fermented feed and tomato pomace, applied in animal feed, animal feed, application, etc., can solve the problem of low application rate of by-products, and achieve the effects of improving utilization rate, improving microbial composition, and increasing feed intake
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Embodiment 1
[0029] 1. Strains and sources
[0030] Lactobacillus acidophilus, strain number: ASI.2686; Bacillus subtilis, strain number: AS1.354; Candida utilis, strain number: AS2.281 and Aspergillus niger, strain number: AS3.316.
[0031] The above four strains were purchased from Guangdong Institute of Microbiology.
[0032] 2. Preparation of composite microorganisms
[0033] Composite microbial strains refer to the mixture of the following four strains of Lactobacillus acidophilus, Bacillus subtilis, Candida utilis and Geotrichum candidum after expanded cultivation, that is, the original species test tube activation--triangle flask expansion-- Seed tank culture - fermenter tank culture.
[0034] The culture conditions are: Bacillus subtilis, constant temperature shaking culture 30-33°C, 200rpm / min; Lactobacillus acidophilus, 35-37°C static culture; Candida utilis and Geotrichum candida, constant temperature shaking culture 25-28°C, 50rpm / min.
[0035] The composite strain obtaine...
Embodiment 2
[0048] 1. Strains and sources
[0049] Lactobacillus acidophilus, strain number: ASI.2686; Bacillus subtilis, strain number: AS1.354; Candida utilis, strain number: AS2.281 and Aspergillus niger, strain number: AS3.316.
[0050] The above four strains were purchased from Guangdong Institute of Microbiology.
[0051] 2. Preparation of composite microorganisms
[0052] Composite microbial strains refer to the mixture of the following four strains of Lactobacillus acidophilus, Bacillus subtilis, Candida utilis and Geotrichum candidum after expanded cultivation, that is, the original species test tube activation--triangle flask expansion-- Seed tank culture - fermenter tank culture.
[0053]The culture conditions are: Bacillus subtilis, constant temperature shaking culture 30-33°C, 200rpm / min; Lactobacillus acidophilus, 35-37°C static culture; Candida utilis and Geotrichum candida, constant temperature shaking culture 25-28°C, 50rpm / min.
[0054] The compound strain obtained ...
Embodiment 3
[0067] 1. Strains and sources
[0068] Lactobacillus acidophilus, strain number: ASI.2686; Bacillus subtilis, strain number: AS1.354; Candida utilis, strain number: AS2.281 and Aspergillus niger, strain number: AS3.316.
[0069] The above four strains were purchased from Guangdong Institute of Microbiology.
[0070] 2. Preparation of composite microorganisms
[0071] Composite microbial strains refer to the mixture of the following four strains of Lactobacillus acidophilus, Bacillus subtilis, Candida utilis and Geotrichum candidum after expanded cultivation, that is, the original species test tube activation--triangle flask expansion-- Seed tank culture - fermenter tank culture.
[0072] The culture conditions are: Bacillus subtilis, constant temperature shaking culture 30-33°C, 200rpm / min; Lactobacillus acidophilus, 35-37°C static culture; Candida utilis and Geotrichum candida, constant temperature shaking culture 25-28°C, 50rpm / min.
[0073] The composite strain obtaine...
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