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Process for producing gloeosporium bacteria

A production process and spore technology, which is applied in the field of producing fungal spores for killing parasitic weeds Cuscuta, can solve the problems of pesticide residues, low killing rate, and poor specificity, and achieve thorough and specific killing Strong, long-lasting effect

Inactive Publication Date: 2010-12-15
易丛琴 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of pesticides to kill dodder, the actual use effect is poor, the killing rate is low, the specificity is poor, and it has adverse effects on the production of crops; and there are pesticide residues, which bring harm to human health and the environment

Method used

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  • Process for producing gloeosporium bacteria
  • Process for producing gloeosporium bacteria

Examples

Experimental program
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Effect test

preparation example Construction

[0023] (1) Preparation of strains; preparation of first-class slant strains:

[0024] Production of potato culture medium: weigh 200 grams after peeling fresh potatoes, cut them into small pieces about half a centimeter square, add them to 1000 ml of water, keep boiling for half an hour after boiling, then filter with two layers of gauze, and the filtrate will replenish water to 1000 ml, add 20 grams of glucose, stir to dissolve the glucose. Then add 20 grams of agar powder, heat to dissolve the agar powder, then divide it into test tubes, and autoclave at 121°C for 20 minutes.

[0025] Or use dodder seed medium: boil 20 grams of dodder seeds in 800 ml of water, keep boiling for half an hour, then filter with two layers of gauze, and the rest of the operation is the same as that of potato medium.

[0026] The length of the slope is preferably 1 / 3-1 / 2 of the test tube. Inoculate Discospora spp. on the slant under sterile conditions, and culture in the dark at 25-27°C for 3 da...

Embodiment 1

[0045] We used the bacterial agent produced by this method on the holly parasitized by dodder in Leting County, Tangshan City, Hebei Province, and the effect was obvious. 5 days after spraying, the dodder withered and died, but the holly was not affected. After 10 days, the holly returned to growth.

Embodiment 2

[0047] The longan plantation under Guangxi Xingye County Agricultural Bureau uses the bacterial agent produced by this method, and the effect is obvious. After 3 days of spraying, most of the dodders were dead. After removing the dead dodders from the longan by hand, the longan returned to normal growth after 5 days.

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Abstract

The invention relates to a process for producing gloeosporium bacteria, which is characterized by comprising the following steps of: (1) preparing strains; (2) preparing a culture medium; (3) mixing the strains prepared by the step (1) and the culture medium prepared by the step (2) and stirring uniformly; (4) packaging the mixture into a kojitray for fermenting and culturing; (5) drying or drying the mixture in the air; (6) crushing the mixture; (7) testing the viable count; and (8) packaging and storing the mixture. The process has the advantages that: the gloeosporium bacteria produced by the method have the characteristics of high specificity of killing south dodder seed, thorough killing, long efficacy and no harm to human bodies, crops and environment, and are a nontoxic, high-efficiency and environmental-friendly biological herbicide.

Description

technical field [0001] The invention relates to a production process of Discochospora, which is suitable for producing fungal spores for killing parasitic weeds Cuscuta. Background technique [0002] Cuscuta is a vicious parasitic weed and is the subject of international plant disease quarantine. More than 200 species of dodder have been found in the world, and more than 10 species have been found in my country, distributed in Xinjiang, Ningxia, Sichuan, Shaanxi, Inner Mongolia, Shanxi, Hebei, Shandong, Jiangsu, Zhejiang, Guangdong, Guangxi, three northeastern provinces, Taiwan and other regions . Cuscuta can parasitize on various crops, horticultural plants and medicinal plants such as cotton, soybean, pepper, sugar beet, tomato, longan, holly, Fufang vine, hazelnut and forsythia. The parasitized plants will reduce yield by 20-30% in mild cases, and even fail to harvest in severe cases. At present, there is only one kind of pesticide registered in our country that can kil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P13/02C12R1/645
Inventor 易丛琴贾佩峤
Owner 易丛琴
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