Drug composition for preventing and treating swine influenza and preparation method and application thereof
A composition and technology of swine flu, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of type B influenza virus ineffectiveness, neurotoxicity, teratogenicity, etc., and improve the prevention of bird flu ability, important social value, and broad economic value
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Embodiment 1
[0054] Embodiment 1: prepare the large volume injection of pharmaceutical composition of the present invention
[0055] Take 1000g of Radix Isatidis, 550g of Forsythia, 250g of Gypsum, 200g of Reed Root, 300g of Rehmannia, 300g of Patchouli, 280g of Iris, 180g of Curcuma, and 180g of Anemarrhena, and process it into herbal decoction pieces, add 8 times the amount of raw material in water, soak After 3 hours, decoct and extract twice, 1 hour each time, combine the extracts, filter, concentrate under reduced pressure to a relative density of 1.25 at 65°C, cool; add ethanol to make the alcohol content to 70%, and let it stand; take The supernatant was recovered and ethanol was removed, concentrated to a relative density of 1.15 at 65°C; countercurrently extracted with 2 times the amount of ethyl acetate, the ester extracts were combined, recovered and ethyl acetate was removed, and the full amount of 50% water for injection was added. Boil, cool, filter, add an appropriate amount...
Embodiment 2
[0056] Embodiment 2: prepare the small volume injection of pharmaceutical composition of the present invention
[0057] Take 700g isatis root, 350g forsythia, 350g gypsum, 300g reed root, 300g rehmannia glutinosa, 50g patchouli, 30g turmeric, 30g anemarrhena, decoct with water, filter, concentrate the filtrate under reduced pressure, add ethanol and let it stand , filtered, concentrated under reduced pressure to 9g drug / ml, diluted with water, refrigerated, filtered, concentrated under reduced pressure, separated with a macroporous resin, rinsed with water, NaOH solution and 35wt% ethanol successively, and then the concentration was 75wt% ethanol solution, collect the eluate; add L-arginine to the eluent diluted with water, adjust the pH to 7.5, add activated carbon, heat and filter for decarbonization, add the same volume of water as the eluent to dilute, add The concentration of L-arginine is 4wt% of the total weight.
Embodiment 3
[0058] Embodiment 3 prepares the 20ml freeze-dried powder injection of pharmaceutical composition of the present invention
[0059] Take 300g of isatidis root, 100g of forsythia, 450g of gypsum, 80g of reed root, 150g of rehmannia glutinosa, 250g of patchouli, 300g of shichangpu, 250g of turmeric, and 600g of anemarrhena, add 3200ml of water for injection and soak for 1 hour, add 6400ml of water for injection and decoct for two minutes. times, each time for 1.5 hours, filter, and combine the filtrates to obtain the extract composition. Take 10g of the extraction composition, add an appropriate amount of water for injection to dissolve it, add 2.25g of sodium chloride, adjust the volume to 250ml with water for injection, add an appropriate amount of charcoal for injection, boil for 20 minutes, let cool, filter, sterilize, and press 1ml body The integral is poured into a low boron glass ampoule, cooled and dried to obtain a freeze-dried powder injection sample.
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