Supercharge Your Innovation With Domain-Expert AI Agents!
Monoclonal antibody of anti-human tissue kallikrein and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A kallikrein and monoclonal antibody technology, applied in the field of immunochemistry, to achieve the effect of strong specificity, low cost and high titer
Inactive Publication Date: 2013-01-16
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
This antibody is a monoclonal antibody against a specific epitope of KLK1. There is no monoclonal antibody against this epitope at home and abroad. It has laid a solid foundation for the development of an efficient ELISA kit for the detection of kallikrein content in human tissues.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0039] Preparation of immunogen (12P-KLH) and coating agent (12P-BSA)
[0040] 12P is a polypeptide composed of 12 amino acids, which is close to the tail of human tissue kallikrein. It has no immunogenicity, only reactogenicity, and cannot directly stimulate animals to produce antibodies. It must be prepared by coupling with macromolecular proteins Adult artificial antigens can induce the body to produce specific antibodies. In this study, 12P was coupled to KLH and BSA by glutaraldehyde method to synthesize the immunogen and the coater.
[0051] Balb / c mice (female, 6 weeks old) were stored in the Experimental Center of Tongji Medical College, Huazhong University of Science and Technology; SP2 / 0 were kept in this laboratory.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
A monoclonalantibody of anti-human tissue kallikrein and a preparation method thereof, wherein the monoclonalantibody of the anti-human tissue kallikrein is generated by the mouse hybridoma cell strain HTK CCTCC-200928; the antibody is a specific monoclonal antibody cell strain aiming at 12-peptide on one segment at the tail end of the anti-human tissue kallikrein, and is generated by inducing the ascites in the mouse body by hybridoma cell strain HTK and purifying. The method includes the following steps: 1) cross-linking the 12-peptide on the tail end of the artificially compounded anti-human tissue kallikrein with KLH and BSA as the immunogen and peridiumgene; 2) immunizing the mouse; 3) screening the mouse with highest serum value; 4) melting the spleen and myeloma cell to obtain the hybridoma cell strain; 5) screening the hybridoma cell strain HTK; 6) expanding and culturing the hybridoma cell and injecting into the mouse abdominal cavity to generate the ascites; 7) purifying the ascites and getting the anti-human tissue kallikrein monoclonal antibody. The monoclonal antibody of the anti-human tissue kallikrein has strong specificity and high value.
Description
technical field [0001] The invention relates to a monoclonal antibody against human tissue kallikrein and a preparation method thereof, belonging to the technical field of immunochemistry. Background technique [0002] Human tissue kallikrein is a large multigene family consisting of 15 members. Only one of the human and animal tissue kallikreins efficiently releases the biologically active kinin from kininogen. In humans the enzyme is called pancreatic / kidney kallikrein, and the new unified designation KLK1 encodes the hk1 protein. hk1 can hydrolyze low-molecular-weight kininogen to generate kinin, and after kinin binds to the bradykinin B2 receptor on the membrane of vascular smooth muscle cells, it can increase NO in vascular smooth muscle, and then relax blood vessels through the cGMP second messenger pathway to achieve The purpose of reducing blood pressure. The content of kallikrein can be seen in both hereditary hypertension and spontaneous hypertension patients. ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/40C12N15/06C12P21/08
Inventor 汪道文张琴
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
Login to View More 
Login to View More