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Benazolin degrading bacteria and application thereof

A technology of herbicide removal and degrading bacteria, which is applied in the field of herbicide degrading bacteria to achieve the effect of solving the problem of wastewater treatment

Inactive Publication Date: 2012-05-09
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic reports on the biodegradation experiments and engineering treatment of the organic heterocyclic herbicide grass-killing production wastewater

Method used

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  • Benazolin degrading bacteria and application thereof
  • Benazolin degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] Examples of the present invention are described below.

[0010] 1. Enrichment of strains

[0011] Sample collection: Sludge collected from the wastewater treatment pool of a certain grass removal factory in Yangzhou;

[0012] Take 5.00g of activated sludge and place it in 100mL of grass-cured with a concentration of 20mg·L -1 In the basal salt medium, 30°C, 150r·min -1 Cultivate on a shaker for 5 days, insert 5% inoculum into fresh basal salt medium, transfer 3 times, and it is determined that the enrichment solution that can degrade more than 90% of herbicide for 3 consecutive generations is active enrichment solution.

[0013] The formula of the basal salt medium is (g·L -1 ): NaCl 1.0, (NH4) 2 SO 4 1.0,K 2 HPO 4 1.5, KH 2 PO 4 0.5, MgSO 4 ·7H 2 O 0.2, deionized water 1000mL, pH7.0, add 1.5%-1.8% agar to the solid medium.

[0014] 2. Isolation and purification of strains

[0015] Take 0.5mL active enrichment solution for gradient dilution, take 10 -2 ...

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Abstract

The invention provides a benazolin degrading bacteria, and belongs to the technical field of waste water treatment. The bacterial strain is gram stain reaction negative bacteria cd-1 and is classified as methyloversatilis universalis. The accession number of Genbank for the gene sequence Genbank of bacteria stain 16S rRNA is GU350457. The major biological property is G-. The thallus is of irregular short rhabditiform with a size of 0.5*0.6mum and no flagella. The results in oxidase test and catalase test are positive; and the results in nitrate reduction, glucose fermentation and indole reaction are negative. After the bacterial strain cd-1 in an inoculum concentration of 5 percent is inoculated into the basic saline culture medium of 100mg / L benazolin for 48 hours, the benazolin is degraded completely. The benazolin degrading bacteria can be prepared as a farm chemical degrading bactericide for solving the problem of waste water treatment in the process of farm chemical production, so that the invention has great application prospect.

Description

1. Technical field [0001] The invention relates to a berazil-degrading bacterium, which belongs to the technical field of waste water treatment. It is to use microorganisms to degrade chemical pesticides and solve the problem of wastewater treatment in the process of pesticide production. 2. Background technology [0002] Benazolin-ethyl, chemical name: ethyl 4-chloro-2-oxobenzothiazol-3-yl acetate, is an organic heterocyclic herbicide. 15% Caobuling EC is a post-emergent herbicide for controlling broad-leaved weeds in rapeseed fields and other dry field crops. The pure product is light yellow crystalline powder with typical sulfur smell, melting point 77.4℃, density about 1.45g·mL -1 . Extremely stable in acidic medium, not easily hydrolyzed. Slightly soluble in water, the solubility at 20°C is 47mg·L -1 , Soluble in most organic solvents. France Viante Crop Science Company registered in my country in 1990 the commodity 10% emulsifiable concentrate and 50% suspending ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/01C02F101/38C02F103/36A62D101/04A62D101/28
Inventor 蔡天明钱丽花陈立伟
Owner NANJING AGRICULTURAL UNIVERSITY