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Curative effect detection of pantothenic acid and ulcer medicament

A technology of pantothenic acid and drugs, applied in the fields of molecular biology and medicine, can solve problems such as the loss of coding exons

Inactive Publication Date: 2010-12-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CYP3A5*5, CYP3A5*6, CYP3A5*7 can also form alternative splices, leading to premature end of coding or loss of exons

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Example Use of detection kit

[0024] Step 1: Extraction of DNA template

[0025] The genomic DNA of oral epithelial cells was extracted by silica gel adsorption method.

[0026] Step 2: Fluorescence quantitative PCR reaction

[0027] Use the fluorescence quantitative PCR set in the detection kit to perform 3 independent fluorescence quantitative PCR reactions, each reaction system is a total volume of 10μl, containing a concentration of 20ng / μl DNA template 2μl, 1μl 10X fluorescence quantitative PCR reaction buffer , 0.1μl 25mM dNTP mixture, 0.6μl 25mM MgCl 2 Solution, 0.025μl (5units / μl) Taq DNA polymerase, 20μM sense primer and antisense primer 0.225μl, 10μM fluorescent probe with VIC and 0.25μl with FAM fluorescent probe, deionized water 5.325μl.

[0028] The reaction was carried out on a PCR amplification machine, and the reaction conditions were 50°C, 2 minutes, 95°C, 10 minutes, and 60 cycles of 95°C, 30 seconds, 60°C, and 1 minute. After the reaction is over, the fluore...

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PUM

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Abstract

The invention discloses a kit for detecting the curative effect of individual pantothenic acid and ulcer medicaments. The kit comprises a specific primer pair and a specific fluorescent probe pair for detecting polymorphism sites of medicament targeted site CYP2C19 gene, CYP3A4 gene and CYP3A5 gene for proton pump inhibitor (PPI) medicaments, a fluorescence quantitative PCR (Polymerase Chain Reaction) conventional assembly, and the like. The kit of the invention is used for assessing the curative effect of individual pantothenic acid and ulcer medicaments by detecting the polymorphism sites of the medicament targeted site CYP2C19 gene, CYP3A4 gene and CYP3A5 gene for PPI medicaments.

Description

Technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the curative effect of individual pantothenic acid and ulcer drugs by detecting the CYP2C19 gene and CYP3A4 target sites of proton pump inhibitor (PPI) drugs. Gene and CYP3A5 gene polymorphism sites to evaluate the efficacy of individual pantothenic acid and ulcer drugs. Background technique [0002] Peptic ulcer (peptic ulcer) mainly refers to chronic ulcers that occur in the stomach and duodenum, namely gastric ulcer (GU) and duodenal ulcer (DU), due to ulcer formation and gastric acid / stomach It is named after the digestion of proteases. The size of the ulcer can vary from a few millimeters to a few centimeters in size. The difference between ulcer and erosion is the depth of penetration: the former has a mucosal defect exceeding the muscularis mucosa; the latter is relatively superficial and does not in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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