Ralstoniasp. and application thereof in bioremediation of petroleum-contaminated saline-alkali soil

A technology of bacterial strains and microbial strains, applied in the restoration of contaminated soil, methods based on microorganisms, microorganisms, etc., can solve the problems of few studies on halophilic bacteria
CN101935631AInactive Publication Date: 2011-01-05BINZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BINZHOU UNIV
Publication Date
2011-01-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a Ralstoniasp. and application thereof in bioremediation of petroleum-contaminated saline-alkali soil. The Ralstoniasp. is collected by China General Microbiological Culture Collection Center (CGMCC), the preservation data is 4 November, 2009, and the preservation number is CGMCCNo.3389. The Ralstoniasp. is grown on an LB solid culture medium to form a white bacterial colony which is round and has regular edge and dry surface and is non-transparent; and the bacterial colony is in a round bar shap, dose not generate spores, is Gram-negative and has the highest growth temperature of 50 DEG C and the NaCl tolerance of 0-5%. The invention also discloses a nucleotide sequence of the Ralstoniasp. The Ralstoniasp. of the invention can degrade pollutants, such as petroleum hydrocarbon, polyaromatic hydrocarbon and the like in soil under the high salt environment; and the crude oil degradation rate can be above 60.0%. Thus, the invention can be applied in the biological control technology of soil pollution brought by polyaromatic hydrocarbon and petroleum.
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Description

technical field

[0001] The invention belongs to the field of microorganisms and their application, and in particular relates to a Ralstonia sp. and its application in bioremediation of oil-contaminated saline-alkali soil. Background technique

[0002] The production and use of oil and its products will inevitably bring about soil pollution problems. For example, about 700 kt of ground crude oil produced by my country's oil companies every year, after recycling, 70 kt still enters the soil environment. Petroleum and petroleum products generally contain toxic and harmful substances such as polycyclic aromatic hydrocarbons (PAHs), benzene, toluene, ethylbenzene, xylene and phenols. Most of these toxic and harmful substances are considered to have "three causes" (carcinogenic, teratogenic, mutagenic) harmful substances, how to control oil pollution, especially the restoration of environmental pollution caused by polycyclic aromatic hydrocarbons, has become a common concern of sc...

Claims

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