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Marker genetically linked to tobamovirus resistance in cucumber and the use thereof

A tobacco mosaic virus and resistance technology, applied in the direction of plant genetic improvement, application, angiosperm/flowering plants, etc., can solve crop loss and other problems, and achieve the effect of avoiding cumbersome and expensive screening methods

Inactive Publication Date: 2011-01-05
ENZA ZADEN BEHEER BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rapid virus spread in greenhouses can lead to severe crop losses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Introduction to the identification of sensitive and resistant cucumber plants (Cucumis sativus L.)

[0045] The following experimental plan was used to classify cucumber plants (Cucumis sativus L.) as susceptible or resistant to cucumber green mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV) infection. At least in the Netherlands, the best time to implement analytics is from September to April.

[0046] plant material

[0047] The tested plants (Cucumis sativus L.), both resistant and sensitive, were sown in medium-sized vermiculites and grown at 24°C, covered with small-sized vermiculites. After 4 to 5 days (cotyledons had just unfolded), the seedlings were transferred to asbestos blocks, and after 2 days the plants were transferred to an isolated greenhouse.

[0048] After each block of 40 individuals, preferably 4 days after placing them in an isolated greenhouse, the sensitivity control Tyria F1 (commercial F1 cucumber variety ...

Embodiment 2

[0055] Example 2: Identification of AFLP markers to identify a QTL for tobacco mosaic virus resistance

[0056] introduce

[0057] In this example, quality trait loci (QTLs) were identified in resistant cucumber plants (Cucumis sativus L.) using a bulk QTL analysis (BQA) performed by Keygene N.V (Wageningen, The Netherlands). A population of 98 individuals was selected for marker development of tobacco mosaic virus resistance. A total of 96 AFLP primer combinations were screened in two pools containing individuals with the "extreme phenotype" showing resistance to tobacco mosaic virus. Subsequently, 5 candidate markers were validated on 12 resistant and 12 sensitive individuals.

[0058] Mark identification and verification

[0059] The leaf materials of 130 plants were obtained: the Cuc1879-01×OK561 population of 98 individuals provided the population for the test, and the inbred line of 30 donor parents Cuc1879-01 and the male line of the population. DNA is isolated and ...

Embodiment 3

[0073]Example 3: Identification of Tobacco Mosaic Virus-Resistant Cucumber Plants (Cucumis sativus L.) Assisted by Molecular Markers

[0074] molecular analysis

[0075] Genetic material was isolated from susceptible and resistant cucumber plants (Cucumis sativus L.), ie, exhibiting one or more symptoms of viral infection as described above, using standard experimental protocols.

[0076] Next, this gene material is digested with an appropriate restriction enzyme (EcoRI / MseI), and after ligation of the linker, the primer pair SEQNo: 1 5-GAC TGC GTA CCA ATT CGT-3' and SEQIDNo: 2 5'-GAT GAG TCC TGA GTA ACA C-3' or primer pair SEQID No: 3 5'-GACTGC GTA CCA ATT CAT-3' and SEQ ID No: 4 5'-GAT GAG TCC TGA GTA ACGT-3' for AFLP nucleic acid amplification.

[0077] The resulting amplification product was sized by electrophoresis. The presence of the AFLP marker in the genes of independent cucumber plants (Cucumis sativus L.) could be detected as absence (-) or presence (+).

[0078]...

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PUM

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Abstract

The present invention relates to a molecular marker genetically linked to, and capable of identifying, a genetic locus in the cucumber plant (Cucumis sativus L.) genome conferring a general resistance against tobamoviruses, and especially against two commercially important pathogenic tobamoviruses, i.e., cucumber green mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV). The present invention further relates to methods for providing a cucumber plant (Cucumis sativus L.), plants, plant parts and fruits with resistance against tobamoviruses, and especially against two commercially important pathogenic tobamoviruses, i.e., cucumber green mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV).

Description

technical field [0001] The present invention relates to a molecular marker genetically linked to and capable of identifying loci in the genome of cucumber plants (Cucumis sativus L.) that confer general resistance to tobacco mosaic virus, in particular to Two commercially important pathogenic tobacco mosaic viruses are resistant, namely, cucumber green mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV). [0002] The present invention also relates to a method for providing resistance to tobacco mosaic virus to cucumber plants (Cucumis sativus L.), plant parts and fruits, in particular to two commercially important pathogenic tobacco mosaic viruses, namely, Cucumis green Methods for resistance to mottle mosaic virus (CGMMV) and cucumber fruit mottle mosaic virus (CFMMV). Background technique [0003] Cucumber plants (ie, plants of the botanical variety Cucumis sativus) belong to the Cucurbitaceae family of Cucurbitaceae, which also includes members of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H5/00A01H5/08
CPCC12Q1/701C12Q1/6895A01H1/04A01H5/08C12Q2600/13C12Q2600/156A01H6/346
Inventor 亚普·马泽雷罗乌布赖特·范·坎彭南内·法贝尔罗纳尔多·威尔特丁克
Owner ENZA ZADEN BEHEER BV
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