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New method for preparing cerebroprotein hydrolysate

A technology of cerebroprotein hydrolyzate and gastric enzyme hydrolyzate, which is applied in the field of medicine, can solve the problems of low free amino acid content and failure to meet national standards, and achieve improved quality controllability, simple and easy preparation method, and clear effect

Inactive Publication Date: 2011-01-12
张树信
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In cerebroprotein hydrolyzate, free amino acids, active polypeptides and sialic acid play a non-negligible role in the recovery of brain dysfunction, but the content of free amino acids in cerebroprotein hydrolyzate obtained by existing preparation methods is very low, especially free amino acids The content of cerebroprotein hydrolyzate is very low and cannot reach the national standard. Many cerebroprotein hydrolyzate products meet the requirements of the specified standard by supplementing exogenous amino acids. Therefore, it is urgent to develop a safe, effective and quality-controllable natural cerebroprotein hydrolyzate that meets the newly revised national standard and has high content of active ingredients.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 Preparation and content determination of cerebroprotein hydrolyzate of the present invention

[0063] One, the preparation of cerebroprotein hydrolyzate of the present invention

[0064] (1) Preparation of gastric enzyme solution: take the pig gastric mucosa and grind the homogenate with a meat grinder, add water for injection at 0.5 times the amount, then add hydrochloric acid, add HCL 20mL per kilogram of gastric mucosa, temperature 47°C, digestion time 3h, that is Obtain crude gastric enzyme liquid, which contains a mixture of various proteolytic enzymes such as pepsin and histone;

[0065] (2) Hydrolysis of gastric enzyme solution: take freshly frozen mammalian brain tissue, slow it down, use water for injection at a ratio of 1:1.5, and homogenize it with a colloid mill. / LHcl to adjust the pH value to 3.5, add gastric enzyme solution and stir evenly, hydrolyze at 40°C, hydrolyze for 16 hours, heat to a slight boil for 10 minutes, filter with 100-mes...

Embodiment 2

[0080] Example 2 Preparation of cerebroprotein hydrolyzate freeze-dried powder injection of the present invention

[0081] prescription:

[0082] Brain protein hydrolyzate solution 3000mL peptide 50mg / ml; sialic acid 1.37mg / ml

[0083] Mannitol 200g

[0084]

[0085] Water for injection add 2250mL

[0086]

[0087] A total of 1500 sticks were prepared

[0088] Process:

[0089] (1) Weigh the prescribed amount of mannitol and add it to water for injection, stir to dissolve, add activated carbon for needles according to 0.1% of the solution amount, stir at 80°C for 30 minutes, filter until the solution is clear, and set aside;

[0090] (2) Take the cerebroprotein hydrolyzate solution of the prescribed amount, mix it with the above solution, add water for injection to 90% of the total solution, adjust the pH value to 7.0, and replenish the water for in...

Embodiment 3

[0094] Embodiment 3 Preparation of cerebroprotein hydrolyzate injection of the present invention

[0095] prescription:

[0096] Brain protein hydrolyzate 6818mL (polypeptide 22.0mg / ml; sialic acid 0.551mg / ml)

[0097] Add 8182mL of water for injection to adjust the pH value to 7.2, filter through a 0.22um microporous membrane, fill and seal with nitrogen,

[0098] Sterilize at 110°C for 30 minutes, light inspection, and packaging. A total of 1500 sticks (10ml) were prepared.

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Abstract

The invention belongs to the technical field of medicines, in particular relates to a new method for preparing a cerebroprotein hydrolysate. The cerebroprotein hydrolysate prepared by the method contains 10 to 12mg / mL active polypeptides and 0.1 to 0.2mg / mL sialic acid and has the advantages of high content of active ingredients, safety and controllability.

Description

1. Technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a new method for preparing cerebroprotein hydrolyzate. 2. Background technology [0002] Brain protein hydrolyzate was first sold by the Austrian EBEWE pharmaceutical company under the trade name of Cerebrolysin. Brain protein hydrolyzate is an aqueous solution of standardized organ-specific amino acid mixture without protein, which contains a variety of essential amino acids, non-essential amino acids, active polypeptides, sialic acid, etc. Memory loss and concentration disorders have good effects on the treatment of organic cerebral psychiatric syndrome, cerebrovascular insufficiency, neurasthenia, children's brain hypoplasia, epilepsy, stroke, encephalitis, concussion, etc. [0003] In cerebroprotein hydrolyzate, free amino acids, active polypeptides and sialic acid play a non-negligible role in the recovery of brain dysfunction, but the content of free amino ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/30A61P25/00
Inventor 王佰荣张树信
Owner 张树信
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