Grass carp thymosin extrasin beta11 gene sequence

A thymosin and grass carp technology, applied in genetic engineering, plant genetic improvement, hormone peptides, etc.

Inactive Publication Date: 2011-01-19
TONGWEI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, β-thymosin is stil...

Method used

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  • Grass carp thymosin extrasin beta11 gene sequence
  • Grass carp thymosin extrasin beta11 gene sequence
  • Grass carp thymosin extrasin beta11 gene sequence

Examples

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Embodiment Construction

[0020] The present invention will be further elaborated below through the following specific embodiments, but the content of the present invention is not limited thereto at all.

[0021] 1. Extraction of total RNA

[0022] Select the experimental material—healthy female grass carp, body length 570mm, about 1.5 winter age (18 months), 2.4kg. Fresh grass carp intestinal tissues were taken out, quickly frozen in liquid nitrogen and stored in a -80°C refrigerator for later use. Total RNA was extracted by one-step method using TRIzol (Invitrogen Corporation), and the quality of total RNA was detected by formaldehyde denaturing gel electrophoresis and DNA / RNA calculator (Qenequant).

[0023] 2. Synthesis of the first strand of cDNA

[0024] Mix 5ug total RNA of grass carp intestinal cells with 1ul (12uM) of reverse transcription primers (5-terminal core primer and linker primer), heat at 70°C for 2 minutes, place on ice immediately, then add 5x buffer, 10mM dNTP mixture, 20mM DTT...

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Abstract

The invention discloses a grass carp thymosin extrasin beta11 gene sequence. A full expression sequence of a grass carp thymosin extrasin beta11 gene is obtained with a PCR (Polymerase Chain Reaction) method by designing primers with a thymosin extrasin beta11 EST sequence of a grass carp intestinal tract cDNA library preserved in the local laboratory used as a template, and combining with a RACE (Rapid Amplification of cDNA Ends) technology. The invention provides important theoretical basis for researching the structural constitution of the grass carp thymosin extrasin beta11 gene, and probing into the function of the grass carp thymosin extrasin beta11 gene in the disease resistant mechanism of grass carps, the position of the grass carp thymosin extrasin beta11 gene in a functional area, and the difference with other species, and plays a pathbreaking role of exploration on establishing a bond between a grass carp genome project and a research on the grass carp disease resistant mechanism.

Description

technical field [0001] The invention relates to the field of gene cloning in molecular biology, in particular to grass carp thymosin β 11 Nucleotide and amino acid sequences of genes. Background technique [0002] β-thymosin (Thymosin β, Tβ) is a kind of small peptide molecule with various knot functions, the molecular weight is about 5KD, and the isoelectric point is between 5 and 7. It is the main actin monomer binding protein in cells , prevent the polymerization of actin monomers to form actin fibers, maintain the dynamic balance of G-actin and F-actin, and play a vital role in the stability of the cytoskeleton. Thymosin β also promotes the differentiation and maturation of T cells by regulating the activity of terminal deoxynucleotidyl transferase (TDT). In addition, thymosin β also has the functions of relieving inflammation and inhibiting cell apoptosis, and plays an important role in the occurrence of tumors. [0003] At present, only the full-length cDNA of Tβ of...

Claims

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Application Information

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IPC IPC(8): C12N15/16C07K14/575
Inventor 徐恒辜文博袁恬府跃军顾继锐吴江
Owner TONGWEI
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