Method for extracting natural antitumor active protein and polypeptide from chickpea bean

A protein and chickpea technology, applied in the field of extracting natural anti-tumor active proteins and peptides, can solve problems such as unclear functional factors

Active Publication Date: 2011-02-02
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chickpea has a long history of medicine, its functional factors are not clear, especially the research gap of biologically active polypeptide compounds

Method used

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  • Method for extracting natural antitumor active protein and polypeptide from chickpea bean
  • Method for extracting natural antitumor active protein and polypeptide from chickpea bean
  • Method for extracting natural antitumor active protein and polypeptide from chickpea bean

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of chickpea watercress:

[0026] Wash 1kg of chickpeas to remove foreign matter, soak for 1h;

[0027] Put the cleaned and soaked chickpeas into the bean sprouts box to germinate. The temperature of the bean sprouts box is 15°C, the water temperature is 20°C, the watering time is 3 seconds, the watering interval is 2 hours, and the germination time is 4 days;

[0028] Separate the sprouts and watercress of 4-day-germinated chickpeas and dry them separately;

[0029] The dried chickpea watercress is crushed, passed through a 10-mesh sieve, and degreased by cold soaking with n-hexane, and the obtained degreased powder is set aside;

[0030] Extraction of proteins and peptides:

[0031] Take 100 grams of defatted chickpea watercress powder and grind it with liquid nitrogen, then use a phosphate buffer solution with a concentration of 0.1mol / L and a pH of 6.0 to extract with stirring at a temperature of 4°C for 2 hours, filter the extract with gauze, and then ...

Embodiment 2

[0036] Preparation of chickpea watercress:

[0037] Wash 1kg of chickpeas to remove foreign matter, soak for 4 hours;

[0038] Put the cleaned and soaked chickpeas into the bean sprouts box to germinate. The temperature of the bean sprouts box is 20°C, the water temperature is 15°C, the watering time is 5 seconds, the watering interval is 6 hours, and the germination time is 3 days;

[0039] Separate the sprouts and watercress of the 3-day-germinated chickpeas and dry them separately;

[0040] The dried chickpea watercress is crushed, passed through a 40-mesh sieve, and degreased by cold soaking with n-hexane, and the obtained degreased powder is set aside;

[0041] Extraction of proteins and peptides:

[0042] Take 100 grams of defatted chickpea watercress powder and grind it with liquid nitrogen, then use a phosphate buffer solution with a concentration of 0.05mol / L and a pH of 7.0 to extract with stirring at a temperature of 4°C for 1 hour, filter the extract with gauze, ...

Embodiment 3

[0047] Preparation of chickpea watercress:

[0048] Wash 1kg of chickpeas to remove foreign matter, soak for 5 hours;

[0049] Put the cleaned and soaked chickpeas into the bean sprouts box to germinate. The temperature of the bean sprouts box is 25°C, the water temperature is 25°C, the watering time is 7 seconds, the watering interval is 4 hours, and the germination is 5 days;

[0050] Separate the sprouts and watercress of the 5-day-germinated chickpeas and dry them separately;

[0051] The dried chickpea watercress is crushed, passed through a 60-mesh sieve, and degreased by cold soaking with n-hexane, and the obtained degreased powder is set aside;

[0052] Extraction of proteins and peptides:

[0053] Take 100 grams of defatted chickpea watercress powder and grind it with liquid nitrogen, then use a phosphate buffer solution with a concentration of 0.07mol / L and a pH of 5.0 to extract with stirring at a temperature of 4°C for 3 hours, filter the extract with gauze, and ...

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Abstract

The invention relates to a method for extracting natural antitumor active protein and polypeptide from chickpea bean. In the method, protein and polypeptide capable of inhibiting the activity of colonic cancer cells can be obtained by the steps of extracting phosphate buffer solution, precipitating ammonium sulfate, desalting, centrifuging and the like. The method is a basic research on bioactive substances in chickpea which is a Uighur medicinal food dual-purpose plant, and provides a basis for developing related products. The method has the advantages that the extraction process is simple, and the protein and the polypeptide can not be deactivated easily. The prepared polypeptide has the advantages of high biological activity, no toxicity and good thermal stability. The method can be easy for production in a large scale, reduces the cost of the extraction process, and is an ideal method for extracting and preparing bioactive protein and polypeptide. The protein and the polypeptide prepared by the method can be used for preparing functional foods or drug additives.

Description

technical field [0001] The invention relates to a method for extracting natural anti-tumor active protein and polypeptide from chickpea watercress. Background technique [0002] At present, tumor has become the second largest killer after cardiovascular and cerebrovascular diseases. Although the current tumor treatment model has made great progress, tumor is still a disease with high morbidity and mortality worldwide. In the United States, cancer is the leading cause of death in people under the age of 85. And the incidence of many tumors, such as skin cancer, prostate cancer, breast cancer, kidney cancer, etc. is still rising. Tumor is a serious threat to human health, and has become one of the important social problems faced by countries all over the world. Finding highly effective and low-toxic anticancer drugs is one of the research hotspots in the world. [0003] However, there is no large-scale commercial production of an anti-tumor active substance at present. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/30
Inventor 阿吉艾克拜尔·艾萨马庆苓阿布力米提·伊力
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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