Composition for inhibiting aging containing extracts from plant buds

A skin aging and extract technology, applied in skin care preparations, cosmetic preparations, cosmetics, etc., can solve the problems of reduced activity, reduced number of epidermal stem cells, hindered production of collagen fibers and elastic fibers, etc., to promote value-added, The effect of improving antioxidant capacity and enhancing activity

Active Publication Date: 2011-02-02
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It is known that wrinkles are formed centering on the above-mentioned disappearing part, and the healing ability of the wound is reduced
Of course, it is not because the number or activity of epidermal stem cells is reduced so that they cannot perform the functions of the skin that the human body has, but from the perspective of cosmetology,

Method used

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  • Composition for inhibiting aging containing extracts from plant buds
  • Composition for inhibiting aging containing extracts from plant buds
  • Composition for inhibiting aging containing extracts from plant buds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] [Example 1] Skin external preparation composition containing sprout extract as an active ingredient

[0048] 1-1. Preparation of sprout extract

[0049] This example relates to the effective eluting of active ingredients in redwood, bilberry, peach, cabbage, cauliflower, sunflower, and kale shoots or germinated plants.

[0050] First, new shoots are harvested from redwood, bilberry, and peach trees in spring to summer, and frozen at minus 20°C to minus 30°C to prevent browning due to enzymatic reactions.

[0051] Next, in the case of cabbage, cauliflower, sunflower, and kale, after immersing the selected seeds in water for about 6 to 10 hours, spread cotton in the culture container, sprinkle the seeds evenly without overlapping, and then germinate. supply water in such a way that the cotton does not dry out. Cover the seeds with a black cloth until about 2 / 3 of the sprouts emerge, then place them in a sunny place. Sprinkle water 1 to 3 times a day with a sprayer, h...

experiment example 1

[0061] [Experimental example 1] Observation of increased activity of skin epidermal stem cells

[0062] In a 24-well plate incubator with 2 × 10 5 keratinocytes isolated from human epidermis were injected into keratinocytes isolated from human epidermis, and cultured in DMEM medium injected with 2% fetal bovine serum. After that, they were replaced with medium containing the sprout extract prepared in Example 1 above at concentrations of 50 and 500 ppm, respectively, and cultured for 48 hours. The negative control group was a group in which no sprout extract was injected into the above-mentioned medium, and a group in which 10% fetal bovine serum was injected into the above-mentioned medium instead of 2% fetal bovine serum was used as a positive control group. Remove the supernatant, after washing twice with phosphate buffer solution, add 50ul of cell lysis buffer (Cell lysis buffer; 20mM Tris-HCl (pH7.4), 250mM NaCl, 1%Triton X-100), stand for 30 minute. After washing twic...

experiment example 2

[0068] [Experimental example 2] Observation of increased proliferation of skin cells by sprout extract

[0069] 2-1. Proliferation of keratinocytes

[0070] Keratinocytes isolated from human epidermis were added at a concentration of 5,000 per well in a 96-well plate culture device, and cultured with KGM (keratinocyte growth medium, Lonza) medium for 24 hours. Thereafter, they were replaced with KBM (keratinocyte basalmedium (keratinocyte basal medium)) medium containing the sprout extract prepared in Example 1 at a concentration of 50 and 500 ppm, respectively, and cultured for 48 hours. WST-1 solution (Roche) was poured therein at 1 / 10 times, and reacted in the incubator at 37 degreeC for 2 hours. The degree of color development at 450 nm was measured on the plate incubator after the culture was completed, and the degree of proliferation of the cells was quantified. The results are shown in Table 3 below.

[0071] table 3

[0072]

[0073] Referring to Table 3 above...

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PUM

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Abstract

The present invention relates to a composition containing extracts of plant sprouts including sequoia sprouts, bilberry sprouts, peach tree sprouts, Korean cabbage sprouts, broccoli sprouts, sunflower sprouts and kale sprouts as active ingredients. The composition of the present invention promotes skin cell growth and survival, and the growth of skin stem cells, thereby inhibiting skin aging and improving the appearance of the skin.

Description

technical field [0001] The present invention relates to a composition for suppressing skin aging containing plant sprout extracts, in particular, to a composition containing extracts from redwood sprouts, bilberry sprouts, peach sprouts, sprouted cabbage, sprouted cauliflower, sprouted sunflower and sprouted kale. A composition for inhibiting skin aging with the sprout extract as an active ingredient. Background technique [0002] A typical example of adult stem cells present in the skin are epidermal stem cells. Epidermal stem cells located at the border of the epidermis and dermis of the skin divide into two, one remains as an epidermal stem cell, and the other continues to divide again and differentiates into an epidermal cell. Therefore, in terms of embryology, the boundary between the epidermis and dermis maintained in the adult becomes flat due to intrinsic aging and photoaging, and its area decreases. As a result, part of the epidermal stem cells disappears. [0003...

Claims

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Application Information

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IPC IPC(8): A61Q19/08A61K8/97
CPCB05C5/02B05C17/00516B05C21/00
Inventor 张希景闵大珍朴元锡郑智彗朴録贤崔贤贞金知贤金志映
Owner AMOREPACIFIC CORP
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