Method for purifying and preparing allergen vaccine

A purification method and allergen technology, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, antibody medical ingredients, etc., can solve the problems of many impurities and achieve the effect of reducing side effects

Inactive Publication Date: 2011-02-16
北京新华联协和药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the defect that the traditional allergen vacc

Method used

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  • Method for purifying and preparing allergen vaccine
  • Method for purifying and preparing allergen vaccine
  • Method for purifying and preparing allergen vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of Artemisia annua Pollen Allergen Vaccine

[0052] Take the qualified Artemisia annua pollen, add acetone at 1:2 (volume ratio) and repeatedly degrease intermittently, until the supernatant solvent that has been left standing is colorless, pour off the solvent, and dry the residue in a fume hood. After degreasing, the pollen cannot remain. solvent. Weigh defatted pollen, add buffer (PH: 8.0200mmolNa 2 HPO 4 NaH 2 PO 4 50mmol NaCl 0.4% phenol), stirred on a magnetic stirrer at 4°C for 24h, and transferred the mixture to a 500ml centrifuge bottle. Centrifuge at 6000rpm at 4°C for 15min, take the supernatant and discard the residue, centrifuge at 8000rpm at 4°C for 30min, take the supernatant and discard the residue, and filter the supernatant with ordinary filter paper for clarification. The clarified extract is ultrafiltered through a 100KD membrane, the filtrate is collected, and the filtrate is then ultrafiltered through a 5KD membrane, an...

Embodiment 2

[0053] Preparation of Example 2 Household Dust Mite Allergen Vaccine

[0054] The pure mite body of dust mite that has passed the inspection is degreased by adding acetone in a ratio of 1:2 and stirring intermittently until the supernatant solvent is colorless, pour off the degreasing solvent, and dry the residue in a fume hood to keep no acetone remaining in the degreasing raw material. Weigh the degreased raw material, add 50mmol NH at a ratio of 1:25 4 HCO 3 , stirred on a magnetic stirrer at 4°C for 24h, and transferred the mixture to a 500ml centrifuge bottle. Centrifuge at 6000 rpm at 4°C for 15 minutes, take the supernatant and discard the residue, and filter the supernatant with ordinary filter paper for clarification. The clarified extract is ultrafiltered with a 100KD membrane, the filtrate is collected, and the filtrate is then ultrafiltered through a 5KD membrane. During the ultrafiltration process, the buffer solution is continuously added until the filtrate is ...

Embodiment 3

[0055] The preparation of embodiment 3 spruce pollen allergen vaccine

[0056] The spruce pollen that passed the inspection was repeatedly degreased by adding ether in a ratio of 1:2 until the supernatant solvent that stood still was colorless, poured off the solvent, and dried the residue in a fume hood. The pollen after degreasing did not leave any degreasing solvent. Weigh defatted pollen, add buffer (PH: 8.0200mmolNa 2 HPO 4 NaH 2 PO 4 50mmol NaCl 0.4% phenol), stirred on a magnetic stirrer at 4°C for 24h, and transferred the mixture to a 500ml centrifuge bottle. Centrifuge at 6000rpm at 4°C for 15min, take the supernatant and discard the residue, centrifuge the supernatant at 8000rpm at 4°C for 30min, take the supernatant and discard the residue, filter the supernatant through ordinary filter paper, and then filter through a 0.45um membrane filter to obtain the clarified stock solution 100kKD membrane ultrafiltration, collect the filtrate, and then pass through the 5...

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Abstract

The invention discloses a method for removing impurity macromolecular and micromolecular substances irrelevant to allergen from activated protein extracting solution containing allergen. The method removes free micromolecular pigments and pigment molecules combined with protein through static electricity and hydrophobicity from the protein extracting solution which is prepared by the traditional technique. The discoloring extracting solution preparation method does not damage the activity and immunologic specificity of allergen. The invention can be used for preparing a high-activity protein extracting solution without macromolecular and micromolecular substances, and the preparation made from the extracting solution has the advantages of high safety, high diagnosis accuracy and high effectiveness for immunotherapy. From another point of view, the protein purification method improves the quality of the raw materials in a chemical essential way, widens the application range of the raw materials, and prepares the attenuated vaccine for immunotherapy.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for purifying an allergen vaccine and a method for preparing the allergen vaccine. Background technique [0002] The allergen vaccine prepared by the existing allergen vaccine purification process contains allergen active ingredients and allergen-free and allergen-independent ingredients. [0003] "Allergen vaccine" refers to an allergen preparation obtained by extracting and purifying the active ingredients of animals or plants with an appropriate solvent. "Allergen products" include biological products of various allergen extracts used for the diagnosis, treatment and prevention of allergic diseases. In the European Pharmacopoeia, allergen products belong to pharmaceutical preparations, which refer to special substances that can cause and / or stimulate allergic diseases in nature. [0004] Since the beginning of the last century, aqueous preparations of pollen...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61P37/08
Inventor 白彩明裴潇竹姜敏任雅丽汤承祁何立峰牛占坡乔秉善
Owner 北京新华联协和药业有限责任公司
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