Paternity testing single nucleotide polymorphism (SNP) label combinations and detection method for Holstein cattle group

Abstract

The invention discloses paternity testing single nucleotide polymorphism (SNP) label combinations for a Holstein cattle group, and one of the combinations contains 28 SNP labels, and the other of the combinations contains 50SNP labels. In the combination, the nucleotide sequences of the forward primers of the 28 SNP labels are shown as SEQ ID NO.23-50 in turn, the nucleotide sequences of the reverse primers of the 28 SNP labels are shown as SEQ ID NO.73-100 in turn, and the nucleotide sequences of the single base extension primers of the 28 SNP labels are shown as SEQ ID NO.123-150 in turn; the nucleotide sequences of the forward primers of the 50 SNP labels are shown as SEQ ID NO.1-50 in turn, the nucleotide sequences of the reverse primers of the 50 SNP labels are shown as SEQ ID NO.51-100, and the nucleotide sequences of the single base extension primers of the 50 SNP labels are shown as SEQ ID NO.101-150. The SNP label combinations used for the Holstein cattle group have accurate results, optimally utilizes a flight mass spectrometry method, can quickly and conveniently detect SNP label polymorphisms with high-flux so as to conclude the parenthood of the cattle group.

Description

technical field [0001] The invention relates to a detection system for Holstein cattle paternity identification using SNP markers, in particular to 28 or 50 marker combinations, primer sequences and a detection method. Background technique [0002] According to literature reports, the average pedigree error rate of cattle herds around the world can reach 11% (Banos et al., 2001). Due to different feeding methods and production management conditions in different countries, the pedigree error rate also has certain differences. cannot be completely avoided. [0003] If the world average 11% pedigree error rate occurs in actual production, the genetic progress of milk production traits in the American dairy herd will be reduced by 12% to 15% (Banos et al., 2001), which will lead to inbreeding coefficient, sire variance Estimates of cross-country genetic correlations etc. are low. The simulation study of Israel and Weller et al. (2000) showed that if the pedigree error rate rea...

AI Technical Summary

Problems solved by technology

If the detection sample is large, the traditional method takes a long time and has a large workload, so it is more suitable to choose high-throughput methods such as mass spectrometry of flight (MALDI-TOF) or chip

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