Method for quickly detecting environmental endocrine disruptors in seawater

A technology of endocrine disruptors and detection methods, applied in the field of marine environmental chemical analysis, can solve problems such as endangering humans and wild animals, affecting human survival and development, endocrine disorders, etc., and achieve good reproducible results

Inactive Publication Date: 2011-05-04
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

A large number of laboratory studies and epidemiological investigations have indicated that the large amount of EDCs in the environment may endanger the normal functions of the endocrine, nervous, immune and other systems of humans and wild animals and lead to endocrine disorders, abnormal growth and development, birth defects, etc. Ser...

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  • Method for quickly detecting environmental endocrine disruptors in seawater
  • Method for quickly detecting environmental endocrine disruptors in seawater
  • Method for quickly detecting environmental endocrine disruptors in seawater

Examples

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Embodiment 1

[0024] (1) Measure 3ml of seawater sample and add it to a 4ml sample bottle with a diaphragm and a lid, use NaCl to configure the concentration of the sample solution to 100g / L, adjust the pH to 5 with 1mol / L hydrochloric acid, and pass it through the extraction head of the solid-phase microextraction device Pierce the diaphragm and immerse the polyamide coating into the sample with a stirring rate of 500rmp, and use ultrasonic-assisted extraction in a constant temperature water bath. , add 100 μL of N,O bis(trimethylsilyl)trifluoroacetamide derivatization reagent to a sample bottle with a septum seal at 25°C, and expose the polyamide coating to the saturated vapor formed by the derivatization reagent Carry out headspace derivatization reaction for 90 minutes;

[0025] (2) Gas chromatography-mass spectrometry analysis

[0026] The chromatographic conditions are: capillary column model HP-5Max (30m×0.25mm×0.25μm), carrier gas is helium; GC inlet uses a glass liner with a diame...

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Abstract

The invention relates to a method for quickly detecting environmental endocrine disruptors (EED) in seawater. The method comprises the following steps of: (1) after a seawater sample is subjected to ion concentration regulation, pH value regulation and water bath ultrasound-assisted extraction, enriching a target compound by adopting a solid-phase microextraction device, and then carrying out headspace derivatization; and (2) after thermal desorption, quantitatively analyzing the EED by using a gas chromatograph mass spectrometer (GCMS). The invention effectively solves the problems of large sampling quantity, long sampling period, difficult sample storage and complicated pretreatment in chemical analysis for testing while analyzing the EED in marine environment investigation, obtains a satisfactory result of the analysis of a selected target compound, has the advantages of small organic solvent dosage, high selectivity to an object to be tested, high extraction speed, high sample recovery rate, and the like, and is suitable for analyzing EEDs with lower contents and complicated structures in the marine environment.

Description

technical field [0001] The invention belongs to the technical field of marine environmental chemical analysis, and in particular relates to a rapid sample pretreatment and detection method for environmental endocrine disruptors in seawater samples. Background technique [0002] The harmful effect of environmental endocrine disrupting chemicals (EDCs) on human and wild animal health has become the focus of global attention. A large number of laboratory studies and epidemiological investigations have indicated that the large amount of EDCs in the environment may endanger the normal functions of the endocrine, nervous, immune and other systems of humans and wild animals and lead to endocrine disorders, abnormal growth and development, birth defects, etc. Thus seriously affecting the survival and development of human beings. Since most EDCs are more polar than traditional pollutants, coupled with their low concentration in the environment and the complexity of the environmental...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/08
Inventor 平仙隐晁敏
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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