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Use of human fat-derived mesenchymal stem cells in treatment of diseases in kidney and ocular fundus

An adipose-derived, stem cell technology, applied in sensory diseases, urinary system diseases, medical raw materials derived from mammals, etc.

Active Publication Date: 2011-05-11
微能生命科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no published literature on the application of hAD-MSCs in the transplantation treatment of fundus diseases.

Method used

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  • Use of human fat-derived mesenchymal stem cells in treatment of diseases in kidney and ocular fundus
  • Use of human fat-derived mesenchymal stem cells in treatment of diseases in kidney and ocular fundus
  • Use of human fat-derived mesenchymal stem cells in treatment of diseases in kidney and ocular fundus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: related experimental methods

[0038] 1.1. Culture and expansion of human adipose-derived MSCs

[0039] Adult adipose tissue was taken, washed repeatedly with D-Hank'S solution, cut into pieces, digested with 0.2% type I collagenase at 37°C for 30 minutes, washed with a large amount of D-Hank'S solution to terminate the action of type I collagenase, centrifuged at 1000r / m, After 10 minutes, filter with a 100-mesh filter, and repeatedly blow with a pipette to make a single-cell suspension, count, and inoculate at a density of 2×10 6 / ml, with complete medium (containing 58% DMEM / F12+40% MCDB-201, 2% fetal calf serum (FCS), 10ng / ml EGF, 10ng / ml PDGF, 1× insulin-transferrin-selenite Acid (Insulin-Transferrin-Selenium, ITS), 1 × linoleic acid-bovine serum albumin (linoleic acid-bovineserum albumin, LA-BSA), 50μM β-mercaptoethanol, 2mM L-glutamine, 100μg / ml penicillin and 100U / ml streptomycin sulfate), placed at 37°C, 5% CO 2 Cultivate in an incubator, chang...

Embodiment 2

[0118] Example 2: Research on the effect and mechanism of human adipose-derived mesenchymal stem cells in renal ischemia-reperfusion model

[0119] 2.1. Morphology and phenotype of cultured hAD-MSCs

[0120] In the primary culture of MSCs isolated from human fat, there are two types of cells, one is fibroblast-like, which grows scatteredly; the other is endothelial-like, which is polygonal and grows tightly. After subculture, the endothelial-like cells gradually decreased, and basically disappeared at the second passage, and all the visual fields were spindle cells ( figure 1 .B). Flow cytometry analysis showed that hAD-MSCs highly expressed CD29, CD44, CD105 and Flk-1, while hematopoietic and endothelial markers (CD31, CD34 and CD45) were negative, and their expression of MHC class II molecules was low ( figure 1 .A). Cell cycle analysis found that most hAD-MSCs were in the G0 / G1 phase ( figure 1 .C). The hAD-MSCs we isolated and cultured have multilineage differentiatio...

Embodiment 3

[0143] Example 3: Effect of hAD-MSCs in retinal degeneration rat model

[0144] 3.1. Pathological evaluation of therapeutic effect after hAD-MSCs transplantation

[0145] In normal rats, the retinal pigment epithelium was intact, the single layer of PRE cells was flat, arranged neatly and continuously, the outer segments of photoreceptor cells were regularly arranged longitudinally, and each layer of the retina was clear. After sodium iodate injection, retinal RPE cells were first damaged. As shown in the figure, the number of RPE cells began to decrease 1 day after injection, and the arrangement of the outer segment layer of photoreceptor cells began to be disordered. hAD-MSCs transplantation group ( Figure 10 .B), compared with the control group ( Figure 10 .A) There is basically no difference in the degree of retinal damage and repair. Three days after sodium iodate tail vein injection, the RPE cells in the control group were further reduced, and the thickness of the p...

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Abstract

The invention relates to the use of human fat-derived mesenchymal stem cells in the treatment of diseases in kidney and ocular fundus. The invention provides the use of the human fat-derived mesenchymal stem cells in cell preparation for treating diseases in kidney and / or ocular fundus, such as acute kidney injury, renal fibrosis, renal tubular epithelial cell damage, retinosis, diabetic retinopathy and retinal tear. The cell preparation can promote the repair of kidney structure and damaged eyeball pathologic structure with good effect.

Description

technical field [0001] The present invention generally relates to the use of mesenchymal stem cells. Specifically, the present invention relates to the use of human adipose-derived mesenchymal stem cells in kidney and / or fundus diseases. Background technique [0002] Mesenchymal stem cells (MSCs) are widely used in the research of various diseases due to their ability of self-renewal and multilineage differentiation. Human adipose-derived MSCs (hAD-MSCs) are easy to obtain, expand and have the same Source MSCs have similar differentiation potential and have become the most popular seed cells in the field of regenerative medicine in recent years. [0003] Acute kidney injury and the resulting renal fibrosis are common clinical pathological processes. Whether it is a variety of primary glomerular diseases, or secondary kidney diseases such as diabetic nephropathy and lupus nephritis, they are closely related to the degree of renal fibrosis. These diseases can cause apoptosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61P13/12A61P27/02A61K35/28
Inventor 赵春华董方田李康华王旭倩杨治坤晁纬静闫曦韩钦于伟鸿赵潺
Owner 微能生命科技集团有限公司
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