Composition for feed, and feed comprising the same
A composition and feed technology, applied in animal feed, animal feed, application, etc., can solve the problems of undisclosed reproducibility, application and optimal content, no preventive effect of swelling, unknown antibacterial effect of cashew nut shell oil, etc.
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Embodiment 1
[0068] Example 1 Composition for powder feed composed of CNSL and oil absorbing agent
[0069] (1) Sample
[0070] The cashew nut shells were obtained from Kashiyuichi Toray Deing Co., Ltd., and the cashew nut shell liquid (CNSL) was squeezed out for use in the following experiments.
[0071] The composition of CNSL was determined by the following method. That is, using HPLC (Waters600, Japan Woots Co., Ltd.), detector (Waters490E, Japan Woots Co., Ltd.), printer (Cromatpack C-R6A, Shimadzu Corporation), chromatography Column (SUPELCOSIL LC18, SUPELCO). Use acetonitrile: water: acetic acid as a solvent of 80:20:1 (amount ratio), and the flow rate is 2 ml / min. Detection was performed at absorbance at 280 nm.
[0072] The cashew nut shell liquid contained 61.8% by mass of anacardic acid, 8.2% by mass of cardanol, and 19.9% by mass of cardanol.
[0073]CNSL is liquid or solid at room temperature, adheres to human skin, is difficult to remove, and causes skin inflammation. ...
Embodiment 2
[0100] Example 2 Powdered Feed Composition Consisting of Crushed Cashew Nut Shells and Oil Absorbing Agent
[0101] (1) Trial production of preparations
[0102] Cashew nut shells were obtained from Kashiyu-Treaking Co., Ltd., and used for the following tests.
[0103] Cashew nut shells and silica (trade name Siprnat 22, product of Degussa Co.) were uniformly pulverized using a pulverizer (Oster Brander, product of Asswan Co., Ltd.) to prepare a composition for powder feed.
[0104] The results are shown in Table 3.
[0105] table 3
[0106]
[0107] (2) Skin irritation
[0108] Evaluation of skin irritation was carried out in the same manner as in Example 1.
[0109] The results are shown in Table 4.
[0110] Table 4
[0111]
[0112] It can be seen from Table 4 that the reagents of Comparative Examples 1-3 and 1-4 showed mild to strong irritation, while the reagents of Experimental Examples 1-4 and 1-5 were not irritating. CNSL was adsorbed inside the silica and...
Embodiment 3
[0113] Embodiment 3CNSL is to Streptococcus bovis ( S. bovis ) antibacterial effect
[0114] In order to investigate the effect of CNSL on Streptococcus bovis ( S. bovis ) antibacterial effect, Staphylococcus aureus ( Staphylococcos aureus ) bovine isolates, Streptococcus bovis ( S. bovis ) DSM20065 strain, Bacillus subtilis ( Bacillus subtilis ) NBRC 3009 strain, Escherichia coli ( Escherichia coli ) ATCC 11303 strain, Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) NBRC 12689 strain and Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) NBRC 10217 strain was cultured at 37° C. for 1 day in Brain Heart Infusion Broth (manufactured by Nissui Pharmaceutical Co., Ltd.). 10 μL of the bacteria were planted in Brain Heart Infusion Broth supplemented with CNSL, cultured at 37° C. for 2 days, and the minimum inhibitory concentration (MIC) was calculated.
[0115] The results are shown in Table 5.
[0116] table 5
[0117]
[0118] Staphylococcus ...
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