Method for extracting high-purity huperzine A and huperzine B from medicinal plant phlegmariurus crutomerianus

A technology for huperzine A and sargassum, applied in the field of extracting high-purity huperzine A and huperzine B, to achieve the effects of short technical route, simplified production process, and increased added value

Active Publication Date: 2011-05-25
湖南本草制药有限责任公司
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  • Description
  • Claims
  • Application Information

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  • Method for extracting high-purity huperzine A and huperzine B from medicinal plant phlegmariurus crutomerianus
  • Method for extracting high-purity huperzine A and huperzine B from medicinal plant phlegmariurus crutomerianus

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Embodiment 1

[0029] Grind and dry 50kg of Cedarus fir, add deionized water twice to make saturated acidic water (PH=5) in the amount of 500L and 400L respectively, heat and stir at 80°C for 2h and 1.5h respectively, filter with a microfilter, Put the obtained filtrate on the ion exchange resin, after adsorbing for 30min, elute with 3BV of column liquid ammonia water, concentrate, adjust the pH value to neutral and let stand for 10h, filter to obtain the filtrate, and concentrate to 1 / 15 of the original volume; the filtrate Separation on a reversed-phase chromatographic column, collecting the liquid in sections to obtain liquid 1 (huperzine A) and liquid 2 (huperzine B); respectively extract with a mixed solution of ethyl acetate and petroleum ether (volume ratio = 1:1) Two liquids to obtain Huperzine A crude product and Huperzine B crude product; respectively add 80% ethanol to Huperzine A and Huperzine B crude products according to their volume ratio, add 100-150 times the amount, heat and...

Embodiment 2

[0032]Grind and dry 100kg of Chinese cedar fir, extract twice and add deionized water to form saturated acid water (PH=5), respectively 900L and 700L, heat and stir at 80°C for 2h and 1.5h, respectively, filter with a microfilter, Put the obtained filtrate on the ion exchange resin, after adsorbing for 30min, elute with 3BV of column liquid ammonia water, concentrate, adjust the pH value to neutral and let stand for 10h, filter to obtain the filtrate, and concentrate to 1 / 15 of the original volume; the filtrate Separation on a reversed-phase chromatographic column, collecting the liquid in sections to obtain liquid 1 (huperzine A) and liquid 2 (huperzine B); respectively extract with a mixed solution of ethyl acetate and petroleum ether (volume ratio = 1:1) Two liquids to obtain Huperzine A crude product and Huperzine B crude product; respectively add 80% ethanol to Huperzine A and Huperzine B crude products according to their volume ratio, add 100-150 times the amount, heat an...

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Abstract

The invention provides a method for extracting high-purity huperzine A and huperzine B from medicinal plant phlegmariurus crutomerianus, which greatly increases the basis for extracting the huperzine A and the huperzine B from new species. The invention discloses an overall method for separating and extracting the high-purity huperzine A and the high-purity huperzine B from the phlegmariurus crutomerianus. Pure products of the huperzine A and the huperzine B are obtained by adopting acid water extraction, passing ion exchange resin, concentrating, leaching, performing reverse-phase column chromatography, decoloring by using activated carbon, crystallizing and recrystallizing. In the overall separation process, requirements on environmental conditions are low, the separation time is short, and the purity is highest. Separation materials are easy to obtain and cheap; a separation operation process is simple and easy to control; the purification efficiency is high by crystallizing and recrystallizing; and used reagents are nontoxic.

Description

Technical field: [0001] The invention relates to a method for extracting high-purity huperzine A and huperzine B from plant medicinal material Cedarus sargassum, belonging to the technical field of natural product chemistry. Background technique: [0002] Huperzine A and Huperzine B are alkaloids extracted from Huperzaceae plants, which are powerful reversible inhibitors of cholinesterase. longer than the latter. It has selective inhibition of true cholinesterase, and the inhibition intensity is thousands of times that of pseudocholinesterase; the inhibition mode is a mixed type of competitive and non-competitive inhibition, which is significantly different from pure competitive inhibitors; It is easy to enter the central nervous system through the blood-brain barrier, and has both central and peripheral therapeutic effects; it has a long effective time; it is well absorbed from the gastrointestinal tract; it has a large safety index and good stability. It is easy to pass ...

Claims

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Application Information

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IPC IPC(8): C07D221/22C07D471/08
Inventor 赵勇彪
Owner 湖南本草制药有限责任公司
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