Method for safely extracting cordyceps sinensis polysaccharide

A technology of Cordyceps polysaccharides and extraction methods, which can be applied to anti-toxins, digestive system, metabolic diseases, etc., can solve food safety hazards and other problems, and achieve good effect, convenient operation, and improved purity

Inactive Publication Date: 2011-05-25
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, n-butanol and chloroform are toxic substances. When functional foods are prepared from the Cordyceps polysaccharide extracted by the Sevage method to remove protein, the residual acetone and chloroform will cause food safety hazards.

Method used

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  • Method for safely extracting cordyceps sinensis polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Extraction: the Cordyceps sinensis sporocarps dried at 60°C are pulverized with a traditional Chinese medicine pulverizer and passed through a 40-mesh sieve, weighed 10 grams of powder, added 100ml of distilled water, extracted at a water temperature of 60°C for 8 hours, centrifuged (3000r / min, 10 minutes) to collect the supernatant, extract the precipitate with distilled water, repeat the extraction three times, and combine to obtain the extract.

[0021] (2) Concentration: Add the extract to the evaporating flask of the rotary evaporator, the vacuum degree is: -0.1MPa, adjust the temperature of the water bath to 60°C, concentrate the extract to one-third of the original volume, and obtain the concentrated of the extract.

[0022] (3) Deproteinization: Add calcium chloride to the concentrated extract to make the concentration 3% (w / v), adjust the pH to 4, shake at room temperature for 30 minutes and then centrifuge to remove the protein precipitate, and then Calc...

Embodiment 2

[0027] (1) Extraction: the Cordyceps sinensis sporocarp that was dried at 60°C was pulverized with a traditional Chinese medicine pulverizer and passed through a 60-mesh sieve, and 10 grams of powder was weighed, added to 200ml of distilled water, extracted at a water temperature of 60°C for 8 hours, and centrifuged (3000r / min , 10 minutes) to collect the supernatant, extract the precipitate with distilled water, repeat the extraction three times, and combine the extracts.

[0028] (2) Concentration: Add the extract to the evaporating flask of the rotary evaporator, the vacuum degree is: -0.1MPa, adjust the temperature of the water bath to 60°C, concentrate the extract to one-third of the original volume, and obtain the concentrated of the extract.

[0029] (3) Deproteinization: Add calcium chloride to the concentrated extract, the mass volume ratio of the added calcium chloride to the extract is 40:1g / L, adjust the pH to 5, shake at room temperature for 60 minutes Centrifuge...

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Abstract

The invention discloses a method for safely extracting cordyceps sinensis polysaccharide. The method comprises the following steps of: leaching cordyceps sinensis by using water, deproteinizing leaching liquor, precipitating by using ethanol, collecting and drying precipitate to obtain a cordyceps sinensis polysaccharide product, wherein the step of deproteinizing the leaching liquor comprises the following steps of: (1) adding calcium chloride into the leaching liquor, adjusting a pH value to 4 to 5, precipitating protein for 30 to 60min, removing protein precipitate so as to obtain protein-removed liquid, wherein the mass volume ratio of the added calcium chloride to the leaching liquor is (30-40):1g / L; and (2) repeating the operation in the step (1) for 1 to 2 times in the protein-removed liquid so as to obtain deproteinized liquid. In the cordyceps sinensis polysaccharide product prepared by the method, the protein residue is about 1 percent, and the polysaccharide purity is over 85 percent; the calcium chloride has low price; the method has good deproteinizing effect, is convenient to operate, can be performed at room temperature, does not destroy the structure of the polysaccharide, and is suitable for industrialized production; and the prepared cordyceps sinensis polysaccharide is safe and nontoxic, so that the harm to food safety is avoided.

Description

Technical field: [0001] The invention belongs to the field of extraction of pharmacologically active components of traditional Chinese medicines, and in particular relates to a safe extraction method for extracting Cordyceps polysaccharides from fruiting bodies of Cordyceps sinensis. Background technique: [0002] Cordyceps sinensis (Cordyceps sinensis), known as the three major supplements together with ginseng and deer antler, is a hot spot in the research field of functional foods because of its rich content of Cordyceps polysaccharides, cordycepin, cordycepic acid and various amino acids, among which the content of Cordyceps polysaccharides is The largest physiologically active substance, it has strong functions of lowering blood sugar, anti-tumor, delaying aging and protecting the liver. A large number of medical experimental studies have shown that Cordyceps polysaccharide can enhance the mononuclear macrophage system and humoral immune function. It can enhance the ph...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P1/16A61P3/10A61P35/00A61P37/04A61P39/06
Inventor 叶克难钟建春张艳
Owner SUN YAT SEN UNIV
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