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Preparation method of organic copper enzyme preparation

A technology for copper enzymes and preparations is applied in the field of preparation of organocopper enzyme preparations, which can solve the problems of complex production process and high production cost, and achieve the effects of simple production process, low production cost and high conversion rate.

Inactive Publication Date: 2013-11-13
滨州市正元畜牧发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing organic copper production process is complicated and the production cost is relatively high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] A preparation method of an organocopper enzyme preparation, the method is as follows: 81 parts by weight of bran, 13 parts by weight of soybean meal, 0.9 part by weight of rice husk powder, 0.6 part by weight of ammonium sulfate, 0.4 part by weight of potassium dihydrogen phosphate, malt 4 parts by weight, the ratio of 110 parts by weight of copper sulfate solution with a mass percentage concentration of 0.9% is mixed with the above materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water. Make its water content be 65% by weight, then, under the condition that pressure is 0.18MPa, temperature is 125 ℃, carry out high temperature and high pressure sterilization for 43 minutes, after sterilization, when above-mentioned material is cooled to 38 ℃, inoculate 2.3% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and cultivated at a temperature of...

Embodiment 2

[0012] A preparation method of an organocopper enzyme preparation, the method is as follows: 85 parts by weight of bran, 11 parts by weight of soybean meal, 1.1 parts by weight of rice husk powder, 0.4 parts by weight of ammonium sulfate, 0.6 parts by weight of potassium dihydrogen phosphate, malt 2 parts by weight, the ratio of 90 parts by weight of copper sulfate solution with a mass percentage concentration of 1.1% is mixed with the above materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water. Make its water content be 55% by weight, then, under the condition that pressure is 0.22MPa, temperature is 115 ℃, carry out high temperature and high pressure sterilization for 46 minutes, after sterilization, when above-mentioned material is cooled to 36 ℃, inoculate 2.7% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and cultivated at a temperature ...

Embodiment 3

[0014] A method for preparing an organocopper enzyme preparation, the method is as follows: 83 parts by weight of bran, 12 parts by weight of soybean meal, 1.0 part by weight of rice husk powder, 0.5 part by weight of ammonium sulfate, 0.5 part by weight of potassium dihydrogen phosphate, malt 3 parts by weight, the ratio of 100 parts by weight of copper sulfate solution with a mass percentage concentration of 1.0% is mixed with the above materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water. Make its water content be 60% by weight, then, under the condition that pressure is 0.20MPa, temperature is 120 ℃, carry out the high temperature and high pressure sterilization of 45 minutes, after sterilization, when above-mentioned material is cooled to 37 ℃, inoculate 2.5% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and cultivated at a temperature ...

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PUM

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Abstract

The invention provides a preparation method of an organic copper enzyme preparation, which comprises the steps of: uniformly mixing raw materials, such as wheat bran, bean pulp, rice husk powder, a copper sulfate solution and the like to prepare a mixture, uniformly stirring, carrying out high-temperature high-pressure sterilization, inoculating a koji tray strain of candida utilis after sterilizing, uniformly stirring and then culturing, then taking materials obtained by the culturing out, inoculating a koji tray strain of aspergillus usamii, uniformly stirring and then subculturing, then taking materials obtained by the subculturing out, inoculating a koji tray strain of bacillus subtilis, uniformly stirring and then culturing for the third time, and drying and crushing materials obtained by the third-time culturing to prepare the organic copper enzyme preparation. The method has the advantages of simple preparation process and low production cost; three times of transformation are continually carried out by adopting the three stains, and inorganic copper is transformed into organic copper with high transformation rate reaching above 98 percent; and the prepared enzyme preparation integrates organic trace elements, an enzyme preparation and probiotics into a whole, has multiple biological functions, and can enhance the immunity of the fed animal.

Description

technical field [0001] The invention provides a method for preparing an organic copper enzyme preparation. Background technique [0002] At present, high doses of copper sulfate are added to some feeds, although this makes the pigs raised have red skin, bright hair and black feces. However, long-term use of inorganic copper such as copper sulfate in the feed will contain inorganic copper in the manure of the animals. After the manure is used to fertilize the farmland, it will lead to the accumulation of copper in the soil and form pollution. Once these feces pollute the water source, it will lead to deterioration of water quality and cause the death of aquatic organisms. Copper toxicity has been reported in sheep when pastures fertilized with manure from animals fed feeds supplemented with high doses of inorganic copper, were grown on pastures fed to sheep. An effective way to solve these problems is to use organic copper. This can not only meet the needs of feeding anima...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/165A23K20/189
Inventor 吴希恩
Owner 滨州市正元畜牧发展有限公司