Application of bayberry extract in the preparation of marine antifouling coatings
A technology of bayberry extract and marine antifouling, applied in antifouling/underwater coatings, coatings, paints containing biocide, etc., to achieve the effect of simple and mature preparation process, easy-to-obtain raw materials, and good antifouling performance
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Embodiment 1
[0013] Example 1 Detection of the inhibitory effect of bayberry extract on marine micro-fouling organisms
[0014] The biological detection model of marine micro-fouling organisms uses marine fouling bacteria, the most important micro-fouling organisms in the ocean. Pseudomonas sp., a marine fouling bacterium, can be purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, or can be isolated from the surface of steel plates immersed in Xiamen Sea by conventional microbial isolation methods.
[0015] Inoculate Pseudomonas in 2216E liquid medium, culture overnight on a shaker, collect bacteria by centrifugation, wash with sterile seawater and make a suspension, the bacterial concentration is about 10 7 ~10 9 cell / ml. Myrica rubra extract was mixed with the prepared bacterial suspension at concentrations of 1, 5, 10, 50 and 100 μg / ml, and added to a petri dish. In addition, the petri dish with only bacterial suspension but no ...
Embodiment 2
[0017] Example 2 Detection of the inhibitory effect of bayberry extract on marine large-scale soft-fouling organisms
[0018] The biological detection model of marine macrosoft-fouling organisms adopts its representative organism, the bryozoan. The adults of Bugulaneritina were collected from fish raft culture cages in the west sea area of Xiamen, and brought back to the laboratory, put them into glass tanks filled with fresh seawater, and artificial light was used to induce planktonic larvae directly above the glass tanks freed. It is the attachment of planktonic larvae that causes the bryozoa to change from planktonic life to attachment life, so inhibiting the attachment of planktonic larvae can prove that the compound has antifouling activity.
[0019] Dissolve bayberry extract in membrane-filtered seawater (filtered through a filter membrane with a pore size of 0.22 μm), set the concentration gradient to 1, 5, 10 and 50 μg / ml, take 10ml of each solution and add it to a ...
Embodiment 3
[0021] Example 3 Detection of the inhibitory effect of bayberry extract on marine large hard fouling organisms
[0022] The biological detection model of marine macrofouling organisms adopts its representative biological barnacle. The adults of Balanus albicostatus were collected from reefs and bridge piers on the coast of Baicheng, Xiamen, and their Venus larvae were cultured in the laboratory. It is the attachment of the larvae that causes the barnacles to switch from a planktonic to an attached life, so inhibition of the attachment of the larvae is evidence of the antifouling activity of the compound.
[0023] Dissolve bayberry extract in membrane-filtered seawater, set the concentration gradient to 0.1, 1, 10, and 50 μg / ml, take 10ml of each solution and add it to a petri dish, and add another 10ml of membrane-filtered seawater to a petri dish, set control group. Three parallel cups were set up for each experimental group and control group, and 30-80 barnacle Venus larva...
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