Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for improving low-nitrogen tolerance character of wheat crops

A crop and wheat technology, applied in the field of crop breeding, can solve problems such as environmental pollution, economic benefit decline, and economic loss

Active Publication Date: 2011-06-29
SHANGHAI ACAD OF AGRI SCI +1
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the large-scale use of nitrogen fertilizer, while promoting the increase and stability of crop production, it has also brought many disadvantages, such as the decline of economic benefits, the reduction of nitrogen utilization rate, environmental pollution, etc., not only caused huge economic losses, but also Seriously pollute the environment and pose a potential threat to human health
Malting barley requires an excellent standard of grain protein content of 10% to 12%. However, in production, nitrogen fertilizer application is often increased or topdressing is applied in the middle and late stages of growth in order to pursue yield. As a result, the grain protein content exceeds the standard, thereby reducing the quality of beer making

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving low-nitrogen tolerance character of wheat crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0136] Embodiment 1: the low-nitrogen stress-resistant cultivation of barley microspores

[0137] "Hua 30" was used as the test material, and the tassels whose microspores were in the early and middle stages of uninucleate development were selected from Daejeon, and put in the refrigerator for 15 days. When inoculating, the tassels were sterilized with a saturated bleach solution for 15 minutes, and washed 3 to 4 times with sterile water. Each test tube received 10 tassels, poured 15ml of mannitol 60g / L, CaCl 2 1.1g / L, MES 0.976g / L extract, use a 3000rpm high-speed disperser to spin cut at a high speed, filter with a 150-mesh screen, and centrifuge the filtrate at a low speed of 800rpm, repeat 3 times, and collect microspores. The microspores were pretreated with the extract at 25°C in the dark for 2 days. Induction medium without KNO 3 and (NH 4 ) 2 SO 4 N 6 The culture medium is basic medium, which is supplemented with 2,4,5-T 1.0mg / L, KT 0.5mg / L, maltose 90g / L, gluta...

Embodiment 2

[0138] Embodiment 2: Acquisition of tolerant low nitrogen regeneration plants

[0139] After 21 days of induction culture, the embryoid bodies were transferred to the differentiation medium. Differentiation medium without KNO 3 and NH 4 NO 4 The 2 / 3MS is the basic medium, which is added with 6-BA 0.5mg / L, KT 1.5mg / L, NAA0.05mg / L and maltose 30g / L, glutamine and hydrolyzed casein each 400mg / L, pH is 5.8. Under the conditions of 25±1° C. and 10-12 hours of light per day, the differentiation stress culture is carried out for 25-30 days until green regenerated plants are differentiated.

Embodiment 3

[0140] Embodiment 3: the rooting culture of tolerance low nitrogen regeneration plant

[0141] The differentiated regenerated plants were selected and transferred to 1 / 2MS rooting and seedling growth medium with pH 5.8 supplemented with 400 mg / L of glutamine and 400 mg / L of hydrolyzed casein, 5.0 mg / L of paclobutrazol (MET) and 20 g / L of sucrose. Under the conditions of 25±1° C. and 10 to 12 hours of light per day, the cultivation of rooted and strong seedlings is carried out for 25 to 30 days.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for improving the low-nitrogen tolerance character of wheat crops. The method comprises the step of applying low-nitrogen stress screening of organic nitrogen to embryoid induction and embryoid differentiation culture stage of microspore in-vitro culture respectively so as to obtain a regeneration plant. All properties of the regeneration plant with improved low-nitrogen tolerance, which is obtained by low-nitrogen stress screening of haploid cell level organic nitrogen, can perform one-time homozygosis, so the efficiency of low-nitrogen tolerance breeding is improved. The implementation method is simple and convenient and easy to grasp, the whole screening process is performed under a controllable condition completely accordant with a lab, so the result istrue and reliable.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, and relates to a method for improving the low-nitrogen tolerance traits of wheat crops by using seeds as the propagation mode. Background technique [0002] Wheat and barley are the two largest cereal crops in the world. Wheat is the main grain crop in my country, and barley is the main raw material for making beer, and it is also an excellent feed. Nitrogen is an essential nutrient element for crop growth. In order to ensure high and stable crop yield, a large amount of nitrogen fertilizer must be applied. In recent years, with the large-scale use of nitrogen fertilizer, while promoting the increase and stability of crop production, it has also brought many disadvantages, such as the decline of economic benefits, the reduction of nitrogen utilization rate, environmental pollution, etc., not only caused huge economic losses, but also Seriously pollute the environment and pose a potential t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄剑华陆瑞菊王亦菲陈志伟何婷
Owner SHANGHAI ACAD OF AGRI SCI