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Surface biological functionalization method for hydrophobic medical high polymer materials

A polymer material and biological functionalization technology, applied in the field of biofunctional modification of the surface of hydrophobic medical polymer materials, can solve the problem of lack of cell recognition sites

Inactive Publication Date: 2011-06-29
SOUTHEAST UNIV
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  • Application Information

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Problems solved by technology

However, like most polymer materials, the surface of PHBV materials is hydrophobic and lacks cell recognition sites

Method used

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  • Surface biological functionalization method for hydrophobic medical high polymer materials
  • Surface biological functionalization method for hydrophobic medical high polymer materials
  • Surface biological functionalization method for hydrophobic medical high polymer materials

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Experimental program
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Embodiment Construction

[0015] 1) The PHBV film was prepared by spin coating method; PHBV was dissolved in chloroform at a concentration of 10 (w / v)%, and stirred evenly by a magnetic stirrer. The PHBV film was prepared on the surface of the glass slide by spin coating: put the clean slide (Φ: 10 mm) in a homogenizer, drop 60 μL of PHBV solution onto the surface of the slide, and spin coat at 4000 rpm for 30 sec. Then put the spin-coated slides with PHBV membrane into a vacuum drying oven at 60°C for overnight drying in vacuum to remove residual chloroform.

[0016] 2) Amino groups were modified on the surface of the PHBV membrane: (a) Amino groups were introduced into the surface by ammonia plasma treatment: the prepared PHBV membrane was placed in a plasma cleaning machine, vacuumed to 7 Pa, and ammonia gas was introduced to 50 Pa, 60 W process 120 sec. Or (b) introduce amino groups on the surface through dopamine: Dissolve 40 mg of dopamine in 20 mL of 10 mM Tris-HCl buffer (pH 8.5) to make a rea...

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Abstract

A major part of medical high polymer materials are hydrophobic and do not have bioactivity, so it is very necessary to perform the biological functionalization modification of the surface of the medical high polymer materials so as to improve the biological compatibility of the materials. By taking poly(hydroxybutyrate-hydroxyvalerate) (PHBV) which is a non-immunogenic biological material for example, the inventor provides the surface biological functionalization method for the hydrophobic medical high polymer materials. Through ammonia plasma treatment or modifying dopamine, amino can be introduced onto the surface of the PHBV, and consequently the surface of the PHBV can be modified with polyethylene glycol molecules (NHS-PEG-MAL) with different functional groups at terminal and short peptides containing arginine-glycine-aspatic acid (RGD). In-vitro cell experiments and protein absorption experiments prove that the biological compatibility of the modified PHBV is improved obviously. The introduced RGD short peptides can promote the growth of the cells on the surface of the material. Meanwhile, the PEG molecules have nonspecific protein absorption resisting capability, and therefore can prevent the generation of inflammation and the formation of thrombi.

Description

technical field [0001] The invention relates to a biofunctional modification method for the surface of a hydrophobic medical polymer material, which belongs to the field of biomedical materials. technical background [0002] In tissue engineering, biomaterials are widely used to fabricate scaffolds. These scaffolds not only provide mechanical support and 3D structure to cells, but also provide signals that induce tissue formation. The structure, morphology, degradation and provision of bioactive sites of materials are all important design parameters, which may affect the proliferation and differentiation of cells. The surface of the material can also be modified with special bioactive substances to improve its biocompatibility. For example, arginine-glycine-aspartic acid (RGD) short peptides are attached to the surface of biomaterials to promote cell adhesion. RGD short peptide widely exists in extracellular matrix proteins (such as fibronectin, collagen and vitronectin, ...

Claims

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Application Information

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IPC IPC(8): C08J7/12C08L67/04A61L27/18A61L27/22A61L31/06
Inventor 黄宁平汪燕艳吕兰欣
Owner SOUTHEAST UNIV
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