Strong-stability moderate-temperature neutral alpha-amylase high-producing bacterium and zymologic property thereof

A strong stability, amylase technology, applied in the direction of enzymes, bacteria, biochemical equipment and methods, etc., can solve the problem that the enzymatic properties of amylase cannot be fully satisfied

Active Publication Date: 2011-07-20
JIANGNAN UNIV
View PDF1 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the 1970s, my country had industrialized production of α-amylase, and successively developed medium-temperature amylase, high-temperature am

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Strong-stability moderate-temperature neutral alpha-amylase high-producing bacterium and zymologic property thereof
  • Strong-stability moderate-temperature neutral alpha-amylase high-producing bacterium and zymologic property thereof
  • Strong-stability moderate-temperature neutral alpha-amylase high-producing bacterium and zymologic property thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Screening of amylase-producing strains

[0034] Primary screening: Dissolve 1g of soil samples around the starch processing plant in 50mL of sterile water, shake well to make a soil suspension, and enrich the spores in a water bath at 80°C for 20 minutes; dilute the soil suspension to different times and take 200μL to spread on On the solid plate of the above-mentioned screening medium, after culturing for 48 hours, pick several single colonies with large hydrolysis circles / diameters for streaking and isolation, and store them in the seed slant medium.

[0035] Re-screening: Shake flask re-screening of several strains obtained from the primary screening. The shake flask medium is the above-mentioned fermentation medium. At 37°C, 250r / min (rotary shaker), culture for 72h, and then measure the enzyme in the fermentation broth live, and the strain with the highest enzyme production was named XLG-1.

Embodiment 2

[0036] Embodiment 2: the mensuration of amylase activity

[0037] The determination of amylase activity adopts the national standard industrial α-amylase preparation method (QB1805.1-93).

[0038] Definition of enzyme activity unit: 1 mL of liquid enzyme, under the conditions of 60°C and pH 6.0, liquefies 1 mg of soluble starch in 1 minute, which is 1 enzyme activity unit, expressed in U / mL.

Embodiment 3

[0039] Embodiment 3: middle temperature neutral amylase enzymatic property

[0040] (1) Effect of temperature on enzyme activity and enzyme thermal stability

[0041] In the same reaction system, the enzyme activity of the amylase was measured at 30-90°C, and the results were as follows: figure 2 As shown: the enzyme exhibits high enzyme activity between 30-85°C, indicating that the enzyme has a wide temperature adaptability range, and its optimum action temperature is 50-70°C. Store the enzyme solution at constant temperature at 40°C, 60°C, and 70°C for different periods of time to measure the residual enzyme activity. The results are as follows: image 3 As shown: 18 hours at 40°C and 60°C, more than 65% of the enzyme activity remains, while only 3% of the enzyme activity remains after 4 hours at 70°C, indicating that the enzyme activity is very stable below 60°C.

[0042] (2) Effect of pH on enzyme activity and acid stability

[0043] In the same reaction system, the en...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biology, and particularly relates to screening of a strong-stability moderate-temperature neutral amylase high-producing Bacillus subtilis XLG-1 and preliminary study on the zymologic property of the enzyme. The strain is preserved at China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.3871. The biological characteristics of the strain are as follows: the strain is in shape of a long pole; and on a starch-screening solid culture medium, the bacterial colony is shaped like a circle with uneven edges, and the lawn is dry and nontransparent and is milk white in color. Under the condition of shake culture for 72 hours at 37 DEG C, the enzyme activity can be up to 2200U/mL; and after ultraviolet-lithium chloride compound induction mutation, the maximum enzyme yield can be up to 6183U/mL. The enzyme can widely used in multiple fields, such as detergent, beer, food, medicine, leather manufacturing, weaving, paper making and other industries.

Description

technical field [0001] The invention relates to the screening of a highly stable mesophilic neutral α-amylase-producing bacterium with industrial application potential and its enzyme-producing properties, belonging to the field of biotechnology. Background technique [0002] α-amylase (α-amylase, EC3.2.1.1), also known as liquefying enzyme, hydrolyzes long-chain starch into short-chain dextrin and reducing sugar. Because the sugar produced is α-type, it is called α-amylase. The α-1,4 glucosidic bond in the starch molecular chain is randomly cut into short-chain dextrins of different lengths, a small amount of maltose and glucose. α-amylase is one of the main enzymes in the process of starch processing. The reaction does not require coenzymes, and the conditions are mild and there are few by-products. Therefore, it is widely used in detergents, beer, food, medicine, leather, textiles and other fields. Among them, α-amylase from microbial sources has become one of the most pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N9/28C12R1/125
Inventor 许正宏张晓梅窦文芳陆震鸣许泓瑜张旦旦李文秀史劲松陈敬华
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap