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Method for improving hydrogen production by photosynthetic bacteria using bubbling process

A technology of photosynthetic bacteria and bubbling, which is applied in the field of using bubbling to improve the hydrogen production of photosynthetic bacteria, which can solve the problems of complex reactor design, low hydrogen production rate, large distance between industrial production and practical application, etc., so as to facilitate metabolism Effects of growth, increasing cell concentration, and reducing product inhibition

Inactive Publication Date: 2011-07-27
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the hydrogen production by photosynthetic bacteria is the same as other biological hydrogen production methods. The main problem is that the hydrogen production rate is low, the reactor design is complicated, and there is a large distance from industrial production and practical application.

Method used

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  • Method for improving hydrogen production by photosynthetic bacteria using bubbling process
  • Method for improving hydrogen production by photosynthetic bacteria using bubbling process
  • Method for improving hydrogen production by photosynthetic bacteria using bubbling process

Examples

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Effect test

Embodiment 1

[0019] In this embodiment, Rhodopseudomonas palustris is used as the object of implementation, and Rhodopseudomonas palustris belongs to purple non-sulfur photosynthetic bacteria.

[0020] Inoculate the photosynthetic bacteria suspension cultured to the logarithmic growth phase into the sterilized medium, put it into the bubbling photobiochemical reactor, cultivate hydrogen production in batches, and collect the gas by drainage method, every 3h The reactor was subjected to Ar bubbling once at a gas flow rate of 0.2 L / min for 5 min each time.

[0021] Such as Figure 1(a) with Figure 1(b) As shown, under the same culture conditions, bubbling has a great influence on the growth and metabolism of photosynthetic bacteria: the average cell concentration of photosynthetic bacteria is higher under the condition of blowing Ar to the reactor at intervals of 3 hours; the pH value decreases after the middle stage of the reaction rising trend.

Embodiment 2

[0023] The reactor was bubbled with Ar every 6 h at a gas flow rate of 0.2 L / min for 5 min each time. The concentration of photosynthetic bacteria and the pH value of the medium change as Figure 3(a) with Figure 3(b) shown.

Embodiment 3

[0025] The reactor was bubbled with Ar every 12 h at a gas flow rate of 0.2 L / min for 5 min each time. The concentration of photosynthetic bacteria and the pH value of the medium change as Figure 3(a) with Figure 3(b) shown.

[0026] Figure 4 It is a comparison chart of the total hydrogen production of photosynthetic bacteria under four conditions. It can be seen from the figure that the hydrogen production of photosynthetic bacteria under bubbling conditions is significantly higher than that under no bubbling conditions, and the bubbling conditions are separated by 3 hours The hydrogen production was the highest, reaching 33 mmol. Figure 5 It is a comparison chart of light energy conversion efficiency and hydrogen production rate of photosynthetic bacteria under four conditions. It can be seen from the figure that the light energy conversion efficiency and hydrogen production rate of photosynthetic bacteria under the condition of bubbling at intervals of 3 hours are m...

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Abstract

The invention discloses a method for improving hydrogen production by photosynthetic bacteria using a bubbling process, which comprises the following steps: inoculating a bacterial suspension of photosynthetic bacteria cultured to a logarithmic growth phase into a sterilized medium, reloading the medium into a bubbling photo-biochemical reactor, and producing hydrogen by culturing in a sequential batch mode; during hydrogen production, bubbling once every 3-12 hours with a gas flow of 0.2 L / min, each for 5 minutes. The invention enhances the hydrogen-producing performance of photosynthetic bacteria by blowing Ar into the bubbling photo-biochemical reactor, so on one hand, the bacterial liquid is stirred and the homogeneous mixing requirement for the substrate and the bacteria is satisfied, and on the other hand, the product gas dissolved in the liquid medium is blown out by the mass transfer effect between the gas and the liquid, which reduces the product inhibition effect during the hydrogen production process and enhances the hydrogen-producing performance of photosynthetic bacteria.

Description

technical field [0001] The invention relates to a method capable of improving the hydrogen production performance of photosynthetic bacteria, which belongs to the field of bioenergy, and in particular to a method for improving the hydrogen production of photosynthetic bacteria by using bubbling. Background technique [0002] Energy is an important material basis for national economic and social development, and one of the foundations for national strategic security. Human society has more and more demands on energy quantity and higher quality requirements. At the same time, with the massive development, use and long-term consumption of fossil energy, serious environmental pollution problems are caused, which is one of the main reasons for atmospheric and other types of environmental pollution and ecological damage. Reducing the application of traditional fossil fuels, developing and using energy resources while protecting the earth's environment and ecology on which we live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 廖强屈晓凡朱恂王永忠李俊王宏丁玉栋叶丁丁陈蓉
Owner CHONGQING UNIV
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