Tissue culture and propagation method and induction medium of tinospora capillipes gagnep

A technology of induction medium and golden fruit group, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as no successful reports, achieve low natural reproduction rate, promote single seedling rooting, solve Effects of dwindling wild resources

Inactive Publication Date: 2011-08-17
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tissue culture rapid propagation is a modern biotechnology, which has been successfully applied to crops such as banana, Luo Han Guo, swee...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Induction of clump buds

[0027] Take the young stems of golden fruit, first wash the surface dirt with detergent, then rinse with running water for 15 minutes, then treat with 75% ethanol on the ultra-clean workbench for 30s, rinse once with sterile water, and then use 0.1% HgCl 2 Sterilize for 10 min, rinse with sterile water for 5 times; blot dry the explants with sterile filter paper, and use MS to add 6-BA 0.46mg / L, NAA 0.24mg / L, GA 3 0.8mg / L, KT 0.15mg / L, pH value adjusted to 4.5 medium, temperature is 26±3℃, light time is 12~14h.d -1 , light intensity 20~30μmol.m -2 .s -1 Under the same conditions, cultured for 22 days, an average of 25.2 times the number of clump buds were obtained. The clump buds were thicker, greener, and grew slightly slower.

[0028] (2) Rooting induction

[0029] When the clump buds grow to 3-4cm, divide the clump buds into small clumps or inoculate single seedlings into the rooting medium. The rooting medium formula is: on MS, add ...

Embodiment 2

[0033] (1) Induction of clump buds

[0034] Take the young stems of golden fruit, first wash the surface dirt with detergent, then rinse with running water for 15 minutes, then treat with 75% ethanol on the ultra-clean workbench for 30s, rinse once with sterile water, and then use 0.1% HgCl 2 Sterilize for 8 min, rinse with sterile water for 5 times; blot dry the explants with sterile filter paper, and use MS to add 6-BA 0.50mg / L, NAA 0.20mg / L, GA 3 1.0mg / L, KT 0.20mg / L, pH value adjusted to 4.7 on the medium, the temperature is 26±3℃, the light time is 12~14h.d -1 , light intensity 20~30μmol.m -2 .s -1 Under the same conditions, cultivated for 20 days, an average of 27 times the number of clump buds were obtained.

[0035] (2) Rooting induction

[0036] When the clump buds grow to 3-4cm, divide the clump buds into small clusters or inoculate single seedlings into the rooting medium. The rooting medium formula is: on MS, add IBA 1.0mg / L+NAA 1.0mg / L to make ;At the temper...

Embodiment 3

[0040] (1) Induction of clump buds

[0041] Take the young stems of golden fruit, first wash the surface dirt with detergent, then rinse with running water for 15 minutes, then treat with 75% ethanol on the ultra-clean workbench for 30s, rinse once with sterile water, and then use 0.1% HgCl 2 Sterilize for 9 min, rinse with sterile water for 5 times; blot dry the explants with sterile filter paper, and use MS to add 6-BA 0.55mg / L, NAA 0.18mg / L, GA 3 1.3mg / L, KT 0.23mg / L, pH value adjusted to 5.0 on the medium, the temperature is 26±3℃, the light time is 12~14h.d -1 , light intensity 20~30μmol.m -2 .s -1Under the condition of culturing for 18 days, an average of 25.8 times the clustered buds were obtained, the clustered buds were thicker, the leaves were dark green, and the growth was faster.

[0042] (2) Rooting induction

[0043] When the clustered shoots grow to 3-4cm, divide the clustered shoots into small clusters or single seedlings and inoculate them on the rooting ...

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PUM

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Abstract

The invention discloses a tissue culture and propagation method and induction medium of tinospora capillipes gagnep. In the invention, MS (Murashige and Skoog), 6-BA (6-Benzylaminopurine), NAA (Naphthylacetic Acid), GA3 (Gibberellin)and KT (kinetin) are taken as the induction medium of the tinospora capillipes gagnep. Cluster buds and action enzymes in body cells of the tinospora capillipes gagnep can be activated by the combination of the four growth hormones under the environment with suitable consistency and subacidity, the germination and the growth of tinospora capillipes gagnep explants are prompted, and the thick cluster buds can be formed after the explants are cultured for 20 days, thus the propagation is increased to 25 times. In the invention, the MS, IBA (Indolebutyric Acid) and the NAA are further taken as a rooting induction medium, thus a single seedling of the tinospora capillipes gagnep effectively prompts to take root and form a new plant. By the tissue culture and propagation method of the tinospora capillipes gagnep by utilizing the two induction mediums, the tinospora capillipes gagnep can be quickly and effectively propagated, the problems that the wild resources of the tinospora capillipes gagnep are reduced day by day and the natural propagation rate is low are solved, and powerful provenance guarantee is provided for deep development of the medicinal value of the tinospora capillipes gagnep.

Description

technical field [0001] The invention relates to the field of plant tissue culture and reproduction, in particular to a tissue culture and reproduction method and an induction medium thereof. Background technique [0002] Golden Fruit Lam (Tinospora capillipes Gagnep.) is an evergreen entwined vine plant of the genus Cystaceae, mainly produced in Sichuan, Hunan, Guangxi, Hubei, Guizhou and other provinces. It is one of the base plants of traditional Chinese medicine, and has the functions of clearing away heat and detoxification, soothing the throat and relieving pain. The medicinal tuberous root of Jinguolan is a variety of medicinal materials recorded in the "Chinese Pharmacopoeia" over the years. Phramenine, kudzurine, and magnolidine. It is widely used in the folk to treat stomach pain. Modern pharmacological research shows that golden fruit has obvious anti-inflammatory and analgesic, antibacterial, anti-tumor, hypoglycemic, anti-stress, anti-ulcer and detoxification e...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 冯世鑫马小军潘丽梅王晓峰闫志刚黄曦
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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