Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Short peptide and application thereof

A short peptide, action technology, applied in the field of biological science and drug carrier, to achieve the effect of enhanced selectivity, low cost and weakened toxicity

Inactive Publication Date: 2013-07-24
HUAZHONG UNIV OF SCI & TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to research, cell-penetrating peptides can carry a variety of substances, including hydrophilic proteins, polypeptides, DNA, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Short peptide and application thereof
  • Short peptide and application thereof
  • Short peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The short peptide of the present invention is a short peptide composed of a cationic cell penetrating peptide, a connecting sequence and a targeting peptide sequence, and is synthesized by solid-phase chemistry. Membrane peptide plays the role of encapsulating plasmid DNA or other drugs, HHHH is the connecting sequence, which plays the role of connecting and eliminating steric hindrance, and LTVSPWY is the targeting peptide, which plays the role of enriching to the target cells and being taken up by them.

[0024] Observe the encapsulation of plasmid DNA by p20:

[0025] First, the plasmid DNA is wrapped with p20, N represents the positive charge of the peptide chain, and P represents the negative charge of the DNA. figure 1 This is the agarose gel electrophoresis picture of p20 and plasmid DNA mixed with different N / P ratios. It can be seen from the figure that the DNA has been basically completely bound since the N / P ratio is 3:1. figure 2 It is the Zeta potential m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a short peptide and application thereof, belonging to the fields of biological science and medicinal carriers. The short peptide consists of a cationic cell penetrating peptide connecting sequence and a targeted peptide sequence, and the amino acid residue sequence of the short peptide is shown as SEQ ID NO.1 in a sequence table. The short peptide can be used for targeted transfection of tumor cells.

Description

technical field [0001] The invention belongs to the field of biological science and drug carrier. Background technique [0002] An important difficulty in gene therapy and other drug treatments at present is the lack of safe and effective carriers that can deliver drugs to specific locations and enter cells to play a role. To achieve this goal, taking gene therapy as an example, researchers have developed a variety of delivery vehicles. Due to the safety and operational complexity of viral vectors, research on non-viral vectors has also received greater attention. Common non-viral vectors include liposomes and cationic polymers. [0003] Liposome is currently one of the gene carriers with the highest delivery efficiency, has good stability to serum, and is easy to use. However, it is highly toxic. In vivo studies have found that it will accumulate in the lungs and cause inflammation, and the particles formed by wrapping with DNA have a large particle size (>500nm), wh...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C12N15/63
Inventor 张玉慧骆清铭龚铖
Owner HUAZHONG UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com