Anti-EGFR (epidemic growth factor receptor) humanized antibody L2-H3 and coding gene and application thereof

A technology encoding genes and antibodies, applied in applications, antibodies, gene therapy, etc., can solve the problems of murine glycosylation pattern, prolong the half-life of antibodies, and have many hypersensitivity reactions, achieve broad application prospects, and ensure the effect of anti-tumor effect.
CN102153650BInactive Publication Date: 2012-07-04INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
Publication Date
2012-07-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an anti-EGFR (epidemic growth factor receptor) humanized antibody L2-H3 and a coding gene and application thereof. The antibody is formed by a light chain and a heavy chain, wherein the heavy chain is formed by connection of a heavy chain variable region and a heavy chain constant region; the amino acid sequence of the heavy chain variable region is shown in the 1st-145th positions in the SEQ ID NO:3; the heavy chain constant region is the heavy chain constant region of a humanized antibody IgG1; and the amino acid sequence of the light chain is shown in the SEQ ID NO:1. The experimental results prove that the antibody has good binding activity and capability of inhibiting growth and migration of the tumor cells; and the affinity of the common anti-EGFR human-mouthchimeric antibody, namely cetuximab in the domestic and foreign markets is 1.1*10<9>M. The humanized antibody disclosed by the invention can better bind with the EGFR, thus ensuring the anti-tumor effect of the humanized antibody. By adopting a method for preparing the antibody, the light chain and the heavy chain can be simultaneously expressed, the expression ratio of the light chain to the heavy chain is closer to 1:1, and the mutually matched double-chain antibody with higher ratio is generated. In conclusion, the antibody and the preparation method have broad application prospects in thefield of tumor prevention and / or treatment.
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Description

technical field

[0001] The invention relates to an anti-EGFR humanized antibody L2-H3 and its coding gene and application. Background technique

[0002] Epidermal growth factor receptor (EGFR), a member of the epidermal growth factor gene (erbB) family, is overexpressed in about 30% of human tumors, especially non-small cell lung cancer, head and neck squamous cell cancer and colorectal cancer. Many studies at home and abroad have shown that antibodies against EGFR can effectively inhibit the EGFR signal transduction pathway by blocking the binding of ligands outside the cell. , especially head and neck squamous cell carcinoma (80% to 100%), colorectal cancer (25% to 77%), non-small cell lung cancer (40% to 80%), etc. have good curative effect. Epidermal growth factor receptor (EGFR) has become one of the most in-depth studies and one of the most concerned targets for tumor therapy. The application of genetic engineering to develop anti-EGFR monoclonal antibodies has becom...

Claims

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