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Method for extracting chondroitin sulfate

A technology of chondroitin sulfate and extraction method, which is applied in the field of deep processing of pig cartilage, can solve the problems of large amount of alkali consumption, large amount of use, and difficulty in purification, and achieve the effects of avoiding residue, non-toxic side effects, and high purity

Inactive Publication Date: 2011-08-17
湖北远成赛创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the dilute alkali enzymolysis process, the amount of dilute alkali solution is large, which makes the amount of alcohol precipitation larger; in the concentrated alkali enzymolysis process, the amount of alkali is large, which brings difficulties to the subsequent purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting chondroitin sulfate, the steps are as follows:

[0021] 1. Weigh 1 kg of commercially available pig nose cartilage, cut off the connective tissue, wash and boil with water to remove grease, then remove the pig nose cartilage from the water, mince it with a meat grinder, add 3 kg of pure water, and adjust When the pH value reaches 8.5, raise the temperature to 45°C, add 20g of bovine trypsin (enzyme activity is 33,000 U / g), stir and enzymolyze at 45°C for 4 hours, and keep the pH value of the reaction system stable at 8.5 during the enzymolysis process;

[0022] 2. Adjust the pH value of the enzymatic solution to 6.8, add 10g of kaolin and 10g of activated carbon powder in sequence, stir and adsorb at 45°C for 1 hour, then filter to remove the kaolin and activated carbon to obtain the filtrate;

[0023] 3. Adjust the pH value of the filtrate to 6.0, then add three times the volume of 95wt% ethanol, stir well and let it stand, remove the supernatan...

Embodiment 2

[0031] A method for extracting chondroitin sulfate, the steps are as follows:

[0032] 1. Weigh 2kg of commercially available pig nose cartilage, cut off the connective tissue, wash and boil with water to remove grease, then remove the pig nose cartilage from the water, mince it with a meat grinder, add 6kg of pure water, adjust When the pH value reaches 8.6, heat up to 46°C, add 40g of bovine trypsin (enzyme activity is 33,000 U / g), stir and enzymolyze at 46°C for 4.2h, and keep the pH value of the reaction system stable at 8.6 during the enzymolysis process;

[0033] 2. Adjust the pH value of the enzymatic solution to 6.9, add 20g of kaolin and 20g of activated carbon powder in sequence, stir and adsorb at 45°C for 1 hour, then filter to remove the kaolin and activated carbon to obtain the filtrate;

[0034] 3. Adjust the pH value of the filtrate to 6.0, then add three times the volume of 95wt% ethanol, stir thoroughly and let it stand, remove the supernatant to obtain 820g ...

Embodiment 3

[0042] A method for extracting chondroitin sulfate, the steps are as follows:

[0043] 1. Weigh 10kg of commercially available pig nose cartilage, cut off the connective tissue, wash and boil with water to remove grease, then remove the pig nose cartilage from the water, mince it with a meat grinder, add 30kg of pure water, adjust When the pH value reaches 8.8, raise the temperature to 48°C, add 200g of bovine trypsin (enzyme activity is 33,000 U / g), stir and enzymolyze at 48°C for 4.5h, keep the pH value of the reaction system stable at 8.8 during the enzymolysis process;

[0044] 2. Adjust the pH value of the enzymolysis solution to 6.9, add 100g of kaolin and 100g of activated carbon powder in sequence, stir and adsorb at 45°C for 1 hour, then filter to remove the kaolin and activated carbon to obtain the filtrate;

[0045] 3. Adjust the pH value of the filtrate to 6.0, then add three times the volume of 95wt% ethanol, stir well and let it stand, remove the supernatant to o...

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PUM

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Abstract

The invention discloses a method for extracting chondroitin sulfate. Pig nose cartilage is used as a main raw material in the method; and fine chondroitin sulfate is obtained by digestion, impurity removal, water distribution, enzymolysis, decolorization, sedimentation, enzymolysis, nano-filtration, sedimentation and drying. The product produced by using the method has high purity, any toxic or harmful substance is not produced, and the obtained product has high safety and can be widely applied to the fields of medicaments, health-care products and the like.

Description

technical field [0001] The method of the invention relates to the technical field of deep processing of pig cartilage, in particular to a method for extracting chondroitin sulfate, which is a method for extracting chondroitin sulfate by using pig nasal cartilage as a raw material through a biological enzymatic hydrolysis technology. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide extracted from animal tissues. It is a kind of glycosaminoglycan, which is formed by connecting D-glucuronic acid and N-acetylgalactosamine with β-1,4-glycosidic bonds. A polysaccharide composed of repeating disaccharide units, and sulfated on the C-4 or C-6 hydroxyl of N-acetylgalactosamine. Chondroitin sulfate has a protective effect on corneal collagen fibers, can promote the growth of fibers in the matrix, enhance permeability, improve blood circulation, accelerate metabolism, promote the absorption of permeate and eliminate inflammation; its polyanion has stron...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61P3/00A61P29/00A61P27/02A61P19/02
Inventor 叶思叶传发张小东
Owner 湖北远成赛创科技有限公司
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