Flounders quintuplet inactivated vaccine and preparation method thereof

A technology of inactivated vaccines and fish, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, bacterial antigen components, etc., can solve the problems of drug resistance of pathogenic bacteria, disease difficulties, increase prevention, etc., and achieve strong pertinence and Representativeness, multi-applicable objects, and effect of reducing the incidence rate

Inactive Publication Date: 2011-08-31
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Application Information

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Problems solved by technology

The long-term use of antibiotics will not only easily lead to drug resistance of pathogenic bacteria and increase the difficulty of s

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  • Flounders quintuplet inactivated vaccine and preparation method thereof
  • Flounders quintuplet inactivated vaccine and preparation method thereof
  • Flounders quintuplet inactivated vaccine and preparation method thereof

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Embodiment Construction

[0024] Describe material of the present invention, method of use and immune effect in detail below by embodiment:

[0025] This implementation case is made according to the following technical plan: conduct epidemiological investigation of flounder and flounder, isolate and purify bacterial pathogens and determine important pathogenic strains for vaccine preparation, conduct formaldehyde inactivation experiments on bacterial strains and optimize inactivation parameters , taking turbot as the object to vaccinate and test the immune index, and test the immune protection rate through the artificial challenge experiment.

[0026] 1. Epidemiological investigation and screening of important pathogens:

[0027] Through the 9-year epidemiological investigation and research on the cultured turbot, flounder, semi-smooth tongue sole and other major flounder species in my country, it was found that fin rot, ascites, enteritis, bulging eyes, scabies, red body and other diseases are the mai...

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Abstract

The invention relates to a flounders quintuplet inactivated vaccine and a preparation method thereof. The main constituents of the vaccine are mixed by the inactivated bacteria of five bacteria such as the vibrio anguillarum, turbot vibrio, the vibro harveyi, the algae dissolving glue vibrio and the edwardsiella tarda. The preparation method comprises the steps of: respectively preparing the five bacteria into 109-1010cfu/mL of vibrio suspension liquid, inactivating the vibrio suspension liquid by formalin, performing the isovolumetric mixing according to the proportion of 1:1:1:1:1 to prepare the flounders quintuplet inactivated vaccine raw liquid, and evenly mixing the flounders quintuplet inactivated vaccine raw liquid with the 15-35mg/mL astragalus polysaccharides adjuvant raw liquid according to the proportion of 1:1. The flounders quintuplet inactivated vaccine is multiple in immunity effects, can be used for effectively preventing the infection of the five bacterial pathogenic bacteria after being vaccinated for once, and provides a basis for the development of the marine fish multi-germinal inactivated vaccine.

Description

Technical field: [0001] The invention belongs to the preparation technology of seawater fish vaccine, which is a technical method for enhancing the immune effect of the vaccine by increasing the species of inactivated fish pathogenic bacteria in the vaccine and adding astragalus polysaccharide. Background technique: [0002] Flounder and flounder are the main species of industrial mariculture in northern my country, and have made important contributions to the economic development of fishery in coastal areas of my country. In recent years, with the rapid expansion of the scale and output of the flounder aquaculture industry, diseases have become one of the main problems faced by aquaculture production. The frequent occurrence of diseases poses a direct threat to the survival rate of breeding, and the economic losses caused have restricted the healthy and stable development of the industry to a certain extent. [0003] The Yellow Sea Fisheries Research Institute of the Chine...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61K39/39A61P31/04A61K39/106A61K39/12
Inventor 张正王印庚邓楠楠廖梅杰王岚荣小军李彬
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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