Pseudomonas fluorescens of endophytic fungi of achnatherum inebrians and application thereof to biological control

A technology of Pseudomonas fluorescens and endophytic bacteria, applied in the field of endophytic bacteria, to achieve the effect of safe and reliable prevention and control, no pollution to the environment, and no harm to human health

Inactive Publication Date: 2011-08-31
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report on the systematic research on the endophytic bacteria of Drunken Horse Grass. Due to the advantages of easy cultivation, easy control, low production cost, and fast growth,

Method used

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  • Pseudomonas fluorescens of endophytic fungi of achnatherum inebrians and application thereof to biological control
  • Pseudomonas fluorescens of endophytic fungi of achnatherum inebrians and application thereof to biological control
  • Pseudomonas fluorescens of endophytic fungi of achnatherum inebrians and application thereof to biological control

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1: Isolation, screening and identification of Claviceps purpurea CGMCC.No3073

[0030] Through the sampling of Zymagrass in Nanshan Mountain of Urumqi, the root, leaf and seed parts of the sample were divided. Sampling at a distance of 10 meters each using a 5-point sampling method (east, south, west, north, center), each point selects one plant to be tested, and each plant has roots, leaves, and seeds (mature period), each Two organs were sampled. Put the sampled organs of different types into food preservation bags and take them back to the laboratory for separation.

[0031] Rinse the sample with sterile water first, then soak with 75% alcohol for 30s, rinse with sterile water, then soak with 0.1% mercury for 1 min, rinse with sterile water three times, add sterile water to grind, and absorb the slurry Coated on beef extract peptone medium, Gao's No. 1 medium, and PDA medium. Beef extract peptone medium and Gao's No. 1 medium are placed in a 37℃ incubator, and P...

Example Embodiment

[0041] Example 2: Isolation, screening and identification of Pseudomona fluorescenss CGMCC.No3026

[0042] Through the sampling, screening, and separation of Urumqi Nanshan Zhema grass, the specific method can be described in Example 1. See attached image 3 , 5

[0043] The obtained GA colony was round with white hyphae. Microscopic observation showed a long rod shape with mesophytic spores. According to the ninth edition of "Bergey's Manual of Systematic Bacterio-logy" ("Bergey, s Manual of Systematic Bacterio-logy") and "Manual of Common Bacterial System Identification", the shape, size, physiological and biochemical reactions of strain GA were tested. The biological characteristics of the invented GA strain are shown in Table 2.

[0044] Table 2: Physiological and biochemical characteristics of the insecticidal active strain GA

[0045]

[0046] GA uses bacterial universal primers 27F and 1492R:

[0047] 27F: (5’AGAGTTTTATCNTGGCTCAG3’)

[0048] 1492R: (5’GGYTACCTTGTTACGACTT3’)

[...

Example Embodiment

[0051] Example 3: The growth factor of Claviceps purpurea CGMCC. No 3073

[0052] The strain PF-2 was connected to PDA medium containing different salt (NaCl) concentration, antibiotic (ampicillin) concentration, and pH, and placed in a 28°C incubator for 7 days. Then the strain PF-2 was connected to the PDA medium and placed in 4℃, 10℃, 15℃, 20℃, 25℃, 30℃, 35℃, 40℃, 45℃, 50℃, 55℃, 60 Cultivate in an incubator at ℃. The results are shown in Table 3.

[0053] Table 3: Effects of temperature, pH, salt and antibiotics on the growth of strain PF-2

[0054] Temperature(℃)

[0055] From Table 3, the strain PF-2 can grow in the environment of temperature 20℃-45℃, pH7-8, NaCl concentration 0.5%-8%, ampicillin concentration 50μg / ml-400μg / ml, especially respectively. It is most suitable for growth when the temperature is 35℃-40℃, pH7, NaCl concentration 7%-8%, and ampicillin concentration 50μg / ml-100μg / ml.

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Abstract

The invention discloses pseudomonas fluorescens containing substances with insecticidal activity CGMCC No. 3026 and application thereof to biological control. By separation, screening and culturing in root of achnatherum inebrians, the pseudomonas fluorescens has the optimum insecticidal pH value of 7 to 8, can keep higher insecticidal activity in the pH range of 7.2 to 7.4 and has the insecticidal activity at the temperature of between 20 and 37 DEG C. The strain of the invention is applied in the field of biological control over damage of aphis.

Description

[0001] The application of the present invention is a divisional application of the invention and creation titled "endophytic bacteria with insecticidal active substances and its application in biocontrol" filed by the applicant on June 4, 2009. The filing date of the original application is 2009 On June 4, 2009, the application number was 200910113352.7, and the title of the invention was "endophytic bacteria with insecticidal active substances and their application in biocontrol". field of invention [0002] The invention relates to the field of biological control, in particular, the invention relates to an endophytic bacterium with insecticidal effect on insect pests such as cotton bollworm, spider mite and locust and application thereof. Background technique [0003] More than 100 years ago, people have discovered that there are microorganisms in the interior of healthy plant tissues, and these microorganisms were later called plant endophytes (Endophytes) in the literatur...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P7/04C12R1/39
Inventor 史应武张雪冰高雁娄恺
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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