Fluorescent latex particles and preparation method thereof

A latex particle and fluorescence technology, applied in the field of fluorescent latex particles and their preparation, can solve the problems of uneven distribution of fluorescent molecules, poor monodispersity, and easy leakage, and achieves clean and clean surface, mild process conditions, and good monodispersity. Effect

Active Publication Date: 2011-09-07
SINOCARE
View PDF12 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is simple to prepare fluorescent latex particles, but the distribution of fluorescent molecules in the particles is not uniform, the combination is not firm, and it is easy to leak out, especially when used in some non-polar solutions, organic dyes are very easy to be leached
[0007] The polymerization method of fluorescent latex particles generally adopts

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent latex particles and preparation method thereof
  • Fluorescent latex particles and preparation method thereof
  • Fluorescent latex particles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] The present invention provides a method for preparing fluorescent latex particles, which includes a) mixing acrylate monomers, fluorescent dyes and ultrapure water in a weight ratio of 1-15:0.004-0.4:100, stirring, heating, and Under the action of the initiator, soap-free emulsion polymerization occurs to obtain the first reaction product;

[0033] b) Filtering and centrifuging the reaction product obtained in step a) to remove the supernatant, and washing the residue after removing the supernatant to obtain fluorescent latex particles.

[0034] The present invention uses soap-free emulsion polymerization. The so-called soap-free emulsion polymerization refers to an emulsion polymerization process in which no emulsifier is added or only a small amount of emulsifier (its concentration is less than the critical micelle concentration CMC) is added during the reaction process, also known as Emulsifier-free emulsion polymerization. The present invention does not add any emulsifie...

Example Embodiment

[0048] Example 1: Carboxyl fluorescent latex particles with a size of about 250nm

[0049] Add 570ml of ultrapure water, 10ml of MMA, 0.02g of rhodamine B, and 18mg of sodium styrene sulfonate into the reaction kettle, mix and stir them for heating, stirring at 150rpm, and blowing nitrogen for 30min~60min to exhaust the dissolved oxygen in the reaction system as much as possible When the temperature rises to 70°C, add 20ml of potassium persulfate to start timing. After 90 minutes of reaction, MAA and MMA are added. The ratio between them is 1:1. The reaction stops after 2 hours. After cooling, the particles are filtered, and then centrifuged with a 50ml centrifuge tube and washed 3 times to obtain fluorescent latex particles.

Example Embodiment

[0050] Example 2: Carboxyl fluorescent latex particles with a size of about 300 nm

[0051] Add 565ml of ultrapure water, 15ml of MMA, 0.02g of rhodamine 6G, 18mg of sodium styrene sulfonate into the reaction kettle, mix and stir them together for heating, stirring at 180rpm, and blowing nitrogen for 30min-60min to exhaust the dissolved oxygen in the reaction system as much as possible Exhausted. When the temperature rose to 70°C, 20ml potassium persulfate was added to start timing. After the reaction proceeded for 120 minutes, MAA and MMA were added, the ratio between them was 1:1, and the reaction stopped after 2 hours. After cooling, the particles are filtered, and then centrifuged with a 50ml centrifuge tube and washed 3 times to obtain fluorescent latex particles.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for preparing fluorescent latex particles, which comprises the following steps of: a) mixing an acrylic ester monomer, a fluorescent dye and ultrapure water in a weight ratio of (1-15):(0.004-0.4):100, stirring, heating, and performing soap-free emulsion polymerization under the action of an initiator to obtain a first reaction product; and b) filtering and centrifuging the reaction product obtained in the step a), removing supernatant, and washing residues left after the supernatant is removed to obtain the fluorescent latex particles. Process conditions in the preparation method are mild, and the prepared fluorescent latex particles have a uniform and controllable particle size and high monodispersity.

Description

technical field [0001] The invention relates to the field of polymer synthesis, and more particularly to a fluorescent latex particle and a preparation method thereof. Background technique [0002] With the continuous development of medical diagnostic technology, the detection limit of target analytes is constantly decreasing, so it is necessary to continuously develop antigen or antibody labeling technology. At present, the labeling technology mainly uses radioactive isotopes, colloidal gold, fluorescent molecules, enzymes, latex particles, etc. They all have their own advantages, but for complex clinical samples, the development of detection technologies such as high-throughput, microquantification, automation, and sensitivity cannot be realized. A polymer material combining fluorescent dye molecules with latex particles provides a powerful means for realizing this detection technology. Because it not only has the high sensitivity of fluorescent molecules, but also has t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08F220/14C08F220/18C08F220/06C08F212/14C08F2/22C08F2/44C09K11/06
Inventor 聂海龙
Owner SINOCARE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap