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Method for preparing cartilage repairing scaffold material

A scaffold material and cartilage repair technology, applied in medical science, prosthesis, etc., can solve the problems of incapable cartilage repair scaffold material, poor cell compatibility, unsatisfactory porosity, etc. Effects of immune rejection, adhesion and growth promotion

Active Publication Date: 2011-09-14
北京科健生物技术有限公司
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

However, due to the limitations of poor biodegradability, poor cytocompatibility, and unsatisfactory porosity, decellularized animal dermis cannot be used as a scaffold material for cartilage repair.

Method used

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  • Method for preparing cartilage repairing scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] a. Preparation of Dermal Matrix

[0017] Select the back skin of a newborn calf / pony, cut the back hair short, and cut the cowhide of suitable size, 8% Na 2 S hair removal, after cleaning, immerse in 0.5mol / L acetic acid for 2 hours, after cleaning with PBS, dry in a fume hood, adjust the thickness of the dermatome according to actual needs, cut the dermis, and then wash the dermal matrix with distilled water, Cut into the actual required size and shape.

[0018] b. Decellularization of Dermal Matrix

[0019] Wash the dermal matrix obtained in step a, immerse it in 0.01% SDS at room temperature, shake it on a horizontal shaker for 4 hours, wash it with PBS solution for 20 times, and then wash it with distilled water for 20 times, and freeze-dry it in a low-temperature freeze dryer for 24 hours. use.

[0020] c. Remodeling of collagen fibers

[0021] Immerse the decellularized dermis (ADM) obtained through step b in 0.5% trypsin, ultrasonicate at 220V, 250W, and 40% ...

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Abstract

The invention discloses a method for preparing a cartilage repairing scaffold material. According to the method, the cartilage repairing scaffold material is prepared by performing cell removing, reconstructing, crosslinking, freeze-drying, surface modifying and other treatment processes on a dermal matrix from animal skin. The scaffold material is made from the dermal matrix and has good biocompatibility, surface activity and plasticity and even pore structure, contains chondroitin sulfuric acid and other substances, can promote the attachment and the growth of cartilage cells and the healing of articular cartilage injury, and can repair joint coloboma efficiently, therefore, the scaffold material is a novel scaffold material which can effectively repair articular cartilage coloboma.

Description

Technical field: [0001] The invention relates to the technical field of medical articular cartilage damage repair materials, in particular to a preparation method of a cartilage repair scaffold material. Background technique: [0002] In the field of orthopedics, articular cartilage defects are very common diseases. Articular cartilage is a single connective tissue without blood vessels, lymphatic vessels, and nerves. It has limited self-repair ability and is difficult to regenerate after injury. After the injury, pain in the joint area and joint movement disorders will occur. Therefore, the repair and treatment of articular cartilage damage has always been a difficult problem in the field of orthopedics. Autologous chondrocyte transplantation, one of the contents of cartilage tissue engineering, is a method for repairing cartilage defects that has only begun in recent years. It is characterized in that: the cartilage tissue is collected from the normal position of the pa...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 孙磊綦惠陈磊冯华田伟
Owner 北京科健生物技术有限公司
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