Application of nucleotide sequence of rDNA (recombinant deoxyribonucleic acid) ITS (internal transcribed spacer)-D3 region in establishment of DNA (deoxyribonucleic acid) bar code identification system for medicinal plants

An ITS-D3, nucleotide sequence technology, applied in the field of identifying medicinal plants using nucleotide sequences, can solve the problems of slow sequence evolution rate, lack of conserved regions, large workload, etc., achieving strong species differentiation ability and saving The effect of measuring time and sequence alignment is accurate
CN102191318AInactive Publication Date: 2011-09-21GUANGZHOU UNIVERSITY OF CHINESE MEDICINE +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
Publication Date
2011-09-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for identifying medicinal plants by utilizing nucleotide sequences, in particular to an application of a nucleotide sequence of an rDNA (recombinant deoxyribonucleic acid) ITS (internal transcribed spacer)-D3 region in establishment of a DNA (deoxyribonucleic acid) bar code identification system for medicinal plants. The application comprises the following steps of: firstly, detecting the nucleotide sequences of the ITS-D3 regions of medicinal plants, and establishing a DNA bar code database; then, detecting the nucleotide sequence of the ITS-D3 region of a sample to be identified; constructing a clustering tree by the detected nucleotide sequence and the nucleotide sequences of the ITS-D3 regions in the DNA bar code database; determining the medicinal plant having the closest genetic relationship with the sample to be identified in the DNA bar code database according to the clustering tree, and comparing the difference between the nucleotide sequences of the ITS-D3 regions of the medicinal plant and the sample to be identified; and then, by taking the name of the species of the medicinal plant having the closest genetic relationship with the sample to be identified in the database as a reference and combining information such as morphological characteristics and the like, determining the name of the species of the sample to be identified. In the invention, the DNA bar codes have the characteristics of good primer universality, accurate sequence comparison and strong species distinguishing capability.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, relates to detection and application methods including nucleic acid, in particular to a method for identifying medicinal plants by using nucleotide sequences. Background technique

[0002] Medicinal plants are the original plants of Chinese medicinal materials, and they are some plant species with health care and medicinal value. According to statistics, my country has 11,146 kinds of medicinal plants (accounting for 87% of all traditional Chinese medicine resources), belonging to 383 families and 2,309 genera. Rapid and accurate identification of these more than 10,000 medicinal plants is an urgent and arduous task for Chinese medicine workers in my country. The existing identification methods of medicinal plants are mainly based on morphological characteristics. By collecting the morphological characteristics of various organs (such as flowers, fruits, seeds, roots, stems, leaves) or tissues of th...

Claims

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