Preparation method of pumpkin seed antioxidant peptide

A technology of antioxidant peptides and pumpkin seeds, applied in the field of preparation of pumpkin seed antioxidant peptides

Inactive Publication Date: 2011-09-28
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is almost no research on pumpkin seed peptides and their physiological activities at home and abroad, and there is no report on the use of pumpkin seed protein antioxidant peptides as antioxidants, which is of great significance to the development of pumpkin seed cake resources.

Method used

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  • Preparation method of pumpkin seed antioxidant peptide
  • Preparation method of pumpkin seed antioxidant peptide
  • Preparation method of pumpkin seed antioxidant peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] A method for preparing pumpkin seed antioxidant peptide, comprising the following steps: (1) Preparation of pumpkin seed protein isolate solution: using defatted pumpkin seed powder as raw material, using alkali-soluble acid precipitation method to obtain pumpkin seed protein isolate, and then adding water to prepare Pumpkin seed protein isolate solution with a mass concentration of 4%, stirred evenly. The preparation method of defatted pumpkin seed powder is as follows: bake the peeled pumpkin seeds in an oven at 60°C for 4 hours to keep the moisture content below 5%, then crush them, and use supercritical CO 2 Extraction technology for degreasing, the degreasing conditions are: extraction kettle pressure 25MPa, extraction temperature 45°C, separation column pressure 10MPa, separation temperature 50°C, CO 2 The flow rate is 2.4-4.2L / h; the process conditions for extracting pumpkin seed protein by alkali-soluble acid precipitation method are: temperature is 40°C, materi...

Embodiment 2

[0136] A method for preparing pumpkin seed antioxidant peptide, comprising the following steps: (1) Preparation of pumpkin seed protein isolate solution: using defatted pumpkin seed powder as raw material, using alkali-soluble acid precipitation method to obtain pumpkin seed protein isolate, and then adding water to prepare The pumpkin seed protein isolate solution with a mass concentration of 5% is stirred evenly. The preparation method of the defatted pumpkin seed powder is as follows: bake the peeled pumpkin seeds in an oven at 60°C for 4 hours to keep the moisture content below 5%, then pulverize and use Supercritical CO 2 Extraction technology for degreasing, the degreasing conditions are: extraction kettle pressure 25MPa, extraction temperature 45°C, separation column pressure 10MPa, separation temperature 50°C, CO 2 The flow rate is 2.4-4.2L / h; the technological conditions for extracting pumpkin seed protein by alkali-soluble acid precipitation are as follows: temperatu...

Embodiment 3

[0143] A method for preparing pumpkin seed antioxidant peptide, comprising the following steps: (1) Preparation of pumpkin seed protein isolate solution: using defatted pumpkin seed powder as raw material, using alkali-soluble acid precipitation method to obtain pumpkin seed protein isolate, and then adding water to prepare The pumpkin seed protein isolate solution with a mass concentration of 8% was stirred evenly, and the preparation method of defatted pumpkin seed powder was the same as in Example 1; the process conditions for extracting pumpkin seed protein by alkali-soluble acid precipitation were: the temperature was 45°C, and the solid-liquid ratio was 1:40, the pH value is 11, and the extraction time is 2.0h; (2) Enzymolysis: heat the pumpkin seed protein isolate solution obtained in step (1) in a boiling water bath for 15 minutes to denature the protein, and adjust the pH to 3. Then add flavor protease 8000U / g, hydrolyze in a water bath at 55 ℃ for 6 hours, keep the pH...

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Abstract

The invention relates to a preparation method of antioxidant peptide and aims to provide a preparation method of pumpkin seed antioxidant peptide with wide sources of raw materials, low cost and high antioxidant activity. The preparation method comprises the following steps of: (1) preparing pumpkin seed isolated protein solution, namely preparing pumpkin seed isolated protein from defatted pumpkin seed powder serving as a raw material by an alkali-solution and acid-isolation method and preparing the pumpkin seed isolated protein solution by adding water; (2) performing enzymolysis, namely heating the pumpkin seed isolated protein solution in a boiling water bath to denature the protein, adjusting the pH value to be 2.5 to 3.5 after cooling, and adding protease with the dose of 5,000 to 8,000 U/g to hydrolyze in a water bath at the temperature of between 45 and 55 DEG C for 2 to 6 hours; and (3) after deactivating enzyme and performing centrifugation, allowing zymolytic solution to pass through ultrafiltration enrichment active peptide, concentrating, freezing and drying to obtain the pumpkin seed antioxidant peptide. The antioxidant peptide has remarkable reducing capacity, high free radical-removing capacity, wide application, remarkable social and economic benefits, optimal enzymolysis process conditions and wide sources of the raw material, increases utilization ratio of the raw material, and utilizes waste materials.

Description

technical field [0001] The invention relates to a preparation method of antioxidant peptide, in particular to a preparation method of pumpkin seed antioxidant peptide. Background technique [0002] With the improvement of people's living standards, various diseases caused by active oxygen and free radicals have seriously endangered human health, and have attracted widespread attention from all walks of life. In recent years, the evaluation and screening of natural resources with strong antioxidant activity has become a new trend in biology, medicine and food science research, and the antioxidant peptides produced by various raw materials have become a research topic at home and abroad because of their low toxicity and high efficiency. hotspots. [0003] At present, the method of obtaining antioxidant peptides by enzymatic hydrolysis of proteins has been greatly developed. Under the action of specific enzymes, proteins from various sources have obtained many peptides with hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/14A23J1/14A23J3/34
Inventor 范三红刘艳荣
Owner SHANXI UNIV
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