Method for deodorizing and decoloring silkworm chrysalis proteins

A technology for silkworm pupa protein and pupa protein, which is applied in the field of deodorization and decolorization of silkworm pupa protein, can solve problems such as safety questioning, activated carbon removal, difficult processing, etc., and achieves the effects of good effect, low processing temperature and simple post-processing.

Inactive Publication Date: 2013-06-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using activated carbon to decolorize and deodorize silkworm chrysalis protein has a good effect, but it is very difficult to separate activated carbon from silkworm chrysalis protein. The high-speed centrifugation at 12000r / min cannot completely remove activated carbon from silkworm chrysalis protein, and subsequent treatment is very difficult; hydrogen peroxide Oxidation decolorization effect is ideal, and H 2 o 2 It has the advantage of being heated and decomposed into water, but its amino acid is severely damaged, and the safety of its product in food is questioned
The organic solvent impregnation method can only remove lipophilic and low-boiling odorous substances, and the effect of decolorization and deodorization is not ideal

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0020] 1. Preparation of silkworm chrysalis protein solution:

[0021] The dried silkworm chrysalis is extracted with petroleum ether to obtain defatted pupae, which is put into a pulverizer to pulverize to obtain defatted pupae powder. Weigh 20g of defatted pupae powder, soak in distilled water for one day, filter and remove the water to remove part of the hydrophilic, smelly and coloring substances, and at the same time soften the pupae protein, then add NaOH solution with a concentration of 0.25mol / L , stirred at 50°C for 5 hours to extract pupae protein. After the reaction, filter the pupae shell and insoluble impurities with gauze to obtain a clear defatted pupae protein extract. Dilute the silkworm pupae protein extract by one time and place it in the refrigerator in spare.

[0022] 2. Deodorization and decolorization of silkworm chrysalis protein:

[0023] 2.1 Treatment of D101 macroporous resin

[0024] Activation of D101 macroporous resin: Add 95% ethanol to the ma...

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PUM

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Abstract

The invention discloses a method for deodorizing and decoloring silkworm chrysalis proteins, which comprises the following steps: extracting chrysalis oil from dry chrysalises after silk reeling with an organic solvent to obtain degreased chrysalises; pulverizing, and washing with water to remove water-soluble pigments and odorous substances; extracting chrysalis proteins with a NaOH solution to obtain a chrysalis protein extraction liquid; adding a resin into the extraction liquid for adsorption to obtain a deodorized and decolorized silkworm chrysalis protein liquid; regulating the isoelectric point of the deodorized and decolorized silkworm chrysalis protein liquid with an acid; standing, filtering and carrying out vacuum-drying to obtain silkworm chrysalis proteins. The method disclosed by the invention is simple and feasible, does not need special equipment, and can be widely applied in the deodorizing and decoloring of silkworm chrysalis proteins in food, feed or cosmetic industry; and the used adsorptive resin can be regenerated for repeated use.

Description

technical field [0001] The invention relates to the technical field of biological protein processing, in particular to a method for deodorizing and decolorizing silkworm chrysalis protein. Background technique [0002] Silkworm chrysalis is the main by-product of the silk industry, and one ton of raw silk can be produced as a by-product of one ton of dried chrysalis. The crude protein content of ordinary dried pupae is about 56-60%, and the oil content is about 30%. Its protein contains 17 kinds of amino acids, 8 kinds of amino acids required for human nutrition are balanced, easy to absorb and utilize, especially methionine exceeds the standard of FAO / WHO, and can provide cheap raw materials for biochemical pharmaceuticals and edible protein. However, the pupal protein produced from silkworm chrysalis is dark brown and has a strong peculiar smell. If it is not removed, even if it is used as animal feed, it will remain in animal meat and poultry eggs, which limits its devel...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07K1/14
Inventor陈芳艳刘吉平王林川
OwnerSOUTH CHINA AGRI UNIV