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Method for extraction and preservation of helicase

A preservation method and helicase technology, which are applied in the field of helicase extraction and effective preservation at normal temperature, can solve the problems of high price of helicase, high preservation conditions, low extraction technology, etc. Long shelf life and high activity

Active Publication Date: 2011-09-28
叶阿彬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction technology of snail enzyme is low, and the storage conditions are high, so the price of snail enzyme remains high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] In a preferred embodiment of the present invention, fresh and live brown cloud agate snails of more than 15 grams are selected and placed in a plastic box with small holes at the bottom. The utilization space of each plastic box is 30%, and the net starvation is more than 5 days. , Wash the snails with 20~30℃ water every morning and evening, stir the plastic box when washing, use the water to wash away the feces and debris attached to the snail shell, carefully check when washing, and remove the dead snails;

[0014] Take 50KG of snails and wash them with a hammer evenly and forcefully break the snail shells on the workbench. After peeling off the digestive glands, squeeze the digestive juice into a clean plastic container under a working environment of 15°C, and finally filter it through a 450 mesh filter. ;

[0015] Separate the collected snail digestion liquid on a centrifuge at 10°C, 8000~10000 rpm for 12 minutes, then pour out the separated upper clarified liquid and di...

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PUM

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Abstract

The invention discloses a method for extraction and preservation of helicase. According to the method, a snail is selected and treated, and the digestive juice of the snail is extracted, filtered and then centrifugalized for refinement, and then subjected to a vacuum freeze-drying treatment so as to be packed and stored, with 9 kinds of enzyme valuable in application effectively extracted from the snail digestive gland. Adoption of the method provided in the invention can realize the volume production of helicase which can be preserved for 10 years at a normal temperature, while helicase products still maintain good activity. Therefore, the method has good economic benefit.

Description

Technical field [0001] The invention relates to a method for extracting snail enzymes from snail digestive glands and effectively storing them at room temperature. Background technique [0002] Snail is a kind of animal that can be raised artificially, and the digestive juice of snail is rich in various enzymes, there are more than 20 kinds in total; snail enzyme is a kind of mixed enzyme, it is the product of snail comprehensive development and utilization, it can be used as dissolving low-grade real The tool enzymes of nuclear microorganisms and plant cell walls are widely used in the research of cell biology and genetic engineering. There are nine kinds of snail enzymes with application value: cellulase, pectinase, A-amylase, 4-BD Mannose endonuclease, BD glucose hydrolase, A-mannase, mannitol oxidase, amino acid transferase and cholinesterase. However, the current low extraction technology of snail enzymes and high storage conditions make the price of snail enzymes high. Su...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/14C12N9/10
Inventor 叶阿彬
Owner 叶阿彬
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