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Efficient preparation process of gamma-amino-n-butyric acid

A technology of aminobutyric acid and preparation process, which is applied in the field of high-efficiency preparation technology of γ-aminobutyric acid, and can solve the problems of high cost and low yield of finished products.

Inactive Publication Date: 2011-09-28
王剑
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, some institutions and individuals at home and abroad are conducting technical research on the synthesis and production of γ-aminobutyric acid using microorganisms (mainly lactic acid bacteria series), but the yield of finished products in experimental research has always been low, making the cost of industrial production relatively high ,, and our research on this technical project has obtained the optimal formula and innovative process. By selecting excellent strains and improving the production formula and production process, the output of γ-aminobutyric acid can be significantly increased, and the cost can be reduced by 20-30%. Significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Lactobacillus hilgardii (Lactobacillus hilgardii) after the optimized and improved MRS medium is graded and expanded, the bacterial liquid is concentrated by centrifugation, then mixed with the suspension of sodium glutamate, and added with 0.25W / V% Tween 80, 0.25mol / L buffer solution, adjust the pH to be 4.5, stir and mix the reaction for 4 days at 35°C, then add sodium glutamate in batches according to the conversion degree of sodium glutamate particles in the reaction mixture, control the pH value and reaction temperature, and Dynamically add defoamer to eliminate foam, continue to react for 3 days, stop the reaction, use electrodialysis desalting method to purify, and centrifuge to separate the bacteria to obtain γ-aminobutyric acid solution with a content of 17.4%, and then purify to obtain γ-aminobutyric acid -Aminobutyric acid product, white powder, purity 98.11%.

[0026] The medium formula was optimized as follows:

[0027] The composition of the optimized MR...

Embodiment 2

[0029]Lactobacillus hilgardii (Lactobacillus hilgardii) after the optimized and improved MRS medium is graded and expanded, the bacterial liquid is concentrated by centrifugation, and then mixed with the suspension of sodium glutamate, adding 0.4W / V% TW80, 0.20mol / L buffer solution, adjust the pH to be 4.0, stir and mix the reaction for 3.5 days at 37°C, then add sodium glutamate in batches according to the conversion degree of sodium glutamate particles in the reaction mixture, control the pH value and reaction temperature, and Dynamically add defoaming agent to eliminate foam, continue to react for 3 days, then stop the reaction, use electrodialysis desalting method to purify, and centrifuge to separate the bacteria to obtain γ-aminobutyric acid solution, the content reaches 22.5%, and then purify it. γ-aminobutyric acid product, white powder, purity 99.1%.

[0030] The medium formula was optimized as follows:

[0031] The composition of the optimized MRS medium is (g / L): ...

Embodiment 3

[0033] Lactobacillus hilgardii (Lactobacillus hilgardii) after the optimized and improved MRS medium is graded and expanded, the bacterial liquid is concentrated by centrifugation, and then mixed with the suspension of sodium glutamate, adding 0.5W / V% TW80, 0.20mol / L buffer solution, adjust the pH to be 5.1, stir and mix the reaction for 2.5 days at 39°C, then add sodium glutamate in batches according to the conversion degree of sodium glutamate particles in the reaction mixture, control the pH value and reaction temperature, and Dynamically add defoamer to eliminate foam, continue to react for 4 days, stop the reaction, use electrodialysis desalting method to purify, and centrifuge to separate the bacteria to obtain γ-aminobutyric acid solution with a content of 23.7%, and then purify to obtain γ-aminobutyric acid -Aminobutyric acid product, white powder, purity 98.3%.

[0034] The medium formula was optimized as follows:

[0035] The composition of the optimized MRS medium...

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Abstract

The invention discloses an efficient preparation process of gamma-amino-n-butyric acid. The process comprises the following steps: Lactobacillushilgardii and the improved and optimized MRS medium are utilized to perform step expansive culture, the bacterium solution is centrifuged and concentrated, the obtained solution is then mixed with the suspension of sodium glutamate, the cell transforming method is adopted to obtain gamma-amino-n-butyric acid solution of which the content of gamma-amino-n-butyric acid is 10-25%, the solution is purified to obtain a gamma-amino-n-butyric acid product which is white powder and of which purity is more than 98%, and dextrin is utilized to prepare the 20% finished product which can be directly used in the raw materials of food and health food. By adjusting the process conditions and optimizing and improving the fermentation culture medium, the preparation cost of gamma-amino-n-butyric acid can be reduced by 20-30% and the production cycle can be shortened by 30%.

Description

technical field [0001] The invention relates to a high-efficiency preparation process of gamma-aminobutyric acid. The method includes transforming sodium glutamate into cells of Lactobacillus hilgardii to produce gamma-aminobutyric acid, and belongs to the field of biotechnology. Background technique [0002] γ-Aminobutyric acid (GABA for short) is a naturally occurring non-protein amino acid and an important inhibitory neurotransmitter in the mammalian central nervous system. About 50% of the central nervous system synapses use GABA as a transmitter. It plays an important role in the human cerebral cortex, hippocampus, thalamus, basal ganglia and cerebellum, and has a regulatory effect on various functions of the body. When there is a lack of GABA in the human body, emotions such as anxiety, restlessness, fatigue, and worry will occur. Generally, people who have been in high-stress groups for a long time, such as people in a competitive environment, athletes, office worker...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/225
Inventor 王健
Owner 王剑
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