Barley beta-glucan preparation method
A technology for glucan and highland barley is applied in the field of highland barley β-glucan preparation, which can solve problems such as affecting the quality of the extract and pollute the environment, and achieve the effects of easy operation, environmental protection and light color
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[0013] Such as figure 1 As shown, a method for preparing highland barley β-glucan of the present invention is characterized in that the method comprises the following steps:
[0014] 1) Cleaning and crushing steps: remove impurities from the highland barley grains, wash and crush them, and pass through a 20-40 mesh sieve;
[0015] 2) Extraction steps: According to the ratio of material to water 1:6~10, gelatinize in water bath at 90°C for 2 hours, add α-amylase for enzymolysis for 30 minutes, filter the filtrate according to 40 mesh, and continue to filter the residue according to the ratio of material to water 1:3~ 6. Water bath at 90°C for 2 hours, filter according to 40 mesh to obtain the second filtrate, and continue to filter the residue according to the material-to-water ratio of 1:2-4, bathe in 90°C for 2 hours, filter to obtain the third filtrate, and combine the filtrates;
[0016] 3) Centrifugal extraction step: centrifuge at 4000 rpm for 10 minutes to remove starch...
Embodiment 1
[0020] The process route of a method for preparing highland barley β-glucan of the present invention is as follows:
[0021] 20—40 mesh, add α-amylase to remove starch
[0022] Highland barley (removing impurities) → Grinding—————————————————————————————————————————————————————————→
[0023] Adjust pH to 4.5, remove protein, ethanol, dry
[0024] Centrifugate————→centrifugation———→Centrant liquid adjusted to PH=7————→ sediment———→β-glucose
[0025] Sampling
[0026] Polycane products——→Separate packaging and sealing.
[0027] The process conditions adopted in the present invention are as follows: remove impurities from the highland barley grains, wash and pulverize them, pass through a 40-mesh sieve, gelatinize in a water bath at 90°C for 2 hours, add α-high temperature resistant amylase for 30 minutes, Filtrate (40 mesh) to obtain the filtrate, continue to use the ratio of material to water 1:3, bathe in 90°C water for 2 hours, filter (40 mesh) to obtain the second filtra...
Embodiment 2
[0030] The process route of a method for preparing highland barley β-glucan of the present invention is as follows:
[0031] 20—40 mesh, add α-amylase to remove starch
[0032] Highland barley (removing impurities) → Grinding—————————————————————————————————————————————————————————→
[0033] Adjust pH to 4.5, remove protein, ethanol, dry
[0034] Centrifugate————→centrifugation———→Centrant liquid adjusted to PH=7————→ sediment———→β-glucose
[0035] Sampling
[0036] Polycane products——→Separate packaging and sealing.
[0037] The process conditions adopted in the present invention are as follows: remove impurities from the highland barley grains, wash and pulverize them, pass through a 40-mesh sieve, and use a material-to-water ratio of 1:8.
[0038] Gelatinize in water bath at 90°C for 2 hours, add α-high temperature-resistant amylase for enzymolysis for 30 minutes, filter (40 mesh) to obtain the filtrate, and continue to filter the residue according to the ratio of mater...
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