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Enterobacter agglomerans strain and application thereof in weeding

A technology of enterobacteriaceae and enterobacteriaceae, applied in the direction of application, herbicides and algicides, bacteria, etc., can solve the problems of slow effect, large amount of herbicide usage, high production and use costs, and achieve crop safety and low cost. Low, environmentally friendly effect

Inactive Publication Date: 2011-10-05
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Phytopathogenic fungal herbicides are biological herbicides that have been studied more, but these herbicides generally have the disadvantages of large usage, slow effect, certain ecological side effects, high production and use costs, and most of them have not yet entered the practical stage

Method used

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  • Enterobacter agglomerans strain and application thereof in weeding
  • Enterobacter agglomerans strain and application thereof in weeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1. A kind of preparation method that contains the liquid herbicide fungus of W5 bacterial strain

[0025] (1) Preparation of fermentation broth

[0026] Improved LB medium formula: peptone 15g / L, glucose 30g / L, natural pH.

[0027] Primary seed culture: Pick a bacterial loop from a fresh plate and inoculate it into a 500mL Erlenmeyer flask filled with 50mL medium, and incubate at 37°C and 100rpm for 10 hours.

[0028] Secondary seed culture: liquid volume 6L (10L tank), inoculation 240mL (4%), 100rpm, air flow 1.2vvm, pressure 0.06MPa, 37 ℃ for 5 hours.

[0029] 200L tank cultivation: liquid volume 150L, ​​inoculation 6L (4%), 150rpm, ventilation volume 1.2vvm, pressure 0.06MPa, culture at 37°C for 24 hours. That is the fermentation broth. As determined by the plate counting method, the bacterial content in the fermentation broth exceeds 3×10 9 CFU / ml.

[0030] (2) Preparation of liquid herbicide

[0031] Put the fermented liquid into a mixing tank, add...

Embodiment 2

[0032] Embodiment 2. The preparation of solid herbicide

[0033] The macroporous resin was pretreated according to the manufacturer's instructions, and then packed into a column (1000 g of macroporous resin, 6 cm in diameter). The fermentation broth was centrifuged at 4000rpm for 20min to remove bacteria, the supernatant flowed through the macroporous resin column at a flow rate of 4mL / min, and the liquid flowing out was collected. Then flush the column with 95% ethanol at a flow rate of 4 mL / min, and collect the ethanol eluate. The eluate is rotary evaporated to dryness under reduced pressure, and then purified by silica gel column and Sephadex LH-20, and the active component obtained is the solid herbicide.

Embodiment 3

[0034] The herbicidal activity of embodiment 3.W5 bacterial strain liquid and solid herbicide bactericide

[0035] The tested weeds are: amaranth, sorrel, southern red creeper, humulus, campanula, snakeberry, cow chickweed, Violaceae plants, ivy, catalpa, Tianhu coriander, Zhuge There are 22 kinds of vegetables, lotus seedlings, green weeds, wheatgrass, small flying canopy, snakehead sausage, multiflora, tianmingjing, weeping grass, mother-in-law and pig yangyang.

[0036] The liquid herbicide and different concentrations of solid herbicide aqueous solutions were sprayed on the leaves of 22 kinds of weeds, and the necrosis of the leaves was observed.

[0037] The leaves of the above 22 kinds of weeds were sprayed with the liquid herbicide for 24 hours, and the edges of the leaves of 6 kinds of weeds wilted and gradually necrotic, namely amaranth, sorrel, cucurbit weeds, campanula, cow chickweed and Humulus Grass. The aqueous solution of solid herbicides has toxic effects on ...

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Abstract

The invention relates to an allelopathic bacteria-enterobacter agglomerans W5 strain and application thereof as a bioherbicide, belonging to the field of microbes applied to agricultural plant protection. The allelopathic bacteria-enterobacter agglomerans W5 strain is preserved in the China general microbiological culture collection center (address: DaTun road, Chaoyang district, Beijing), with a preserving number of CGMCC No. 4565. The invention also relates to a liquid weeding bacterial agent and a solid weeding bacterial agent containing the allelopathic bacteria-enterobacter agglomerans W5 strain as effective components and applications thereof in controlling dicotyledonous weeds of a farmland or a lawn.

Description

1. Technical field [0001] The invention relates to an enterobacteriaceae strain and its application in weeding, and belongs to the technical field of microorganisms applied to agricultural plant protection and crop weed control, in particular to the control of dicotyledonous weeds in farmland and lawn. 2. Background technology [0002] Weeds are biological disasters that seriously threaten modern agricultural production. Current measures for weed control are mainly through the use of chemically synthesized herbicides and the cultivation of genetically modified plants. The former generally has problems such as poor environmental compatibility and drug resistance, while the latter is limited in use due to the limited number of transgenic varieties and narrow herbicidal spectrum. Therefore, the research and development of highly efficient, environmentally friendly and harmless biological herbicides is undoubtedly the most important and urgent task at present, and the research ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P13/00C12R1/01
Inventor 刘常宏双惊雷郭利川薛雅蓉赵宗政
Owner NANJING UNIV
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