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Method for producing saccharifying enzyme by using beta-cyclodextrin fermentation waste liquor

A technology of fermentation waste liquid and glucoamylase, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problem of high DE value of waste liquid, reduce environmental pressure, save treatment, and save production costs Effect

Inactive Publication Date: 2011-10-05
HENAN UNIV OF SCI & TECH
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Problems solved by technology

[0005] Problems encountered in the production process of β-cyclodextrin: β-cyclodextrin production waste liquid is rich in organic carbon, and the DE value of waste liquid is as high as 15%

Method used

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Embodiment 1

[0026] The following is a specific embodiment of the method for producing glucoamylase using β-cyclodextrin fermentation waste liquid of the present invention.

[0027] 1. Preparation of liquid seeds

[0028] Under aseptic conditions, use a sterile pipette to aspirate 0.1 ml of thawed mycospheres containing 10 3 spores / mL of Aspergillus niger liquid spread on the starch plate culture medium, 34 ° C constant temperature culture for 5 days; then pick 1-2 colonies with regular shape and regular inner depression on the starch medium, and place them in the test chamber. Stretch and separate on Petri dish medium, culture at 34°C for 5 days to make it mature, and store it in a refrigerator at 0-4°C after maturity; prepare Chase semi-dry medium, take it out with an inoculation spatula or a loop after sterilization A single colony that has matured on the petri dish culture medium was placed in the sterilized Chase semi-dry body, the liquid volume was 2 / 5, and the constant temperature ...

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Abstract

The invention provides a method for producing saccharifying enzyme by using beta-cyclodextrin fermentation waste liquor, In the method, aspergillus niger fermentation liquor is prepared by taking a liquid strain containing asporous aspergillus niger as a strain; and the method comprises the following steps of: inoculating the liquid strain in an amount which is 15 percent of the liquid volume into an aspergillus niger culture medium for fermentation, and performing gradual amplification culture by using a culture medium containing the beta-cyclodextrin fermentation waste liquor under certain fermentation conditions to obtain the aspergillus niger fermentation liquor which can directly serve as a saccharifying enzyme product. The beta-cyclodextrin fermentation waste liquor partially replaces starch for production, the production cost is saved and enterprise profit is increased; and the waste liquor is reutilized, the waste is changed into treasure, the treatment of the waste liquor is avoided and the environmental protection pressure is lightened.

Description

[0001] technical field [0002] The invention relates to a biological fermentation technology, in particular to a method for producing glucoamylase by using β-cyclodextrin fermentation waste liquid. Background technique [0003] Glucoamylase (abbreviated GA or G) is the abbreviation of Glucoamylase (Glucoamylase EC 3.2.1.3), also known as γ-amylase, is one of the most important industrial enzyme preparations, it is an exoglycosidase, from The non-reducing end of starch hydrolyzes α-1,4 glycosidic bonds in turn, cuts off each glucose unit, and, like β-amylase, changes the configuration of the hydrolyzed glucose to form β-D-glucose. As for amylopectin, when it encounters a branch point, it can also hydrolyze the α-1,6 glycosidic bond, thereby fully hydrolyzing the amylopectin to glucose. [0004] Glucoamylase is one of the most widely used enzymes in industry, and it is the most produced enzyme variety in China. The main use is as a starch saccharifying agent, so it is widel...

Claims

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Application Information

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IPC IPC(8): C12R1/685C12N9/34
Inventor 赵启美何佳孙晓菲张宏森张占洋李爱江关随霞
Owner HENAN UNIV OF SCI & TECH
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