Method for producing saccharifying enzyme by using beta-cyclodextrin fermentation waste liquor

A technology of fermentation waste liquid and glucoamylase, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problem of high DE value of waste liquid, reduce environmental pressure, save treatment, and save production costs Effect

Inactive Publication Date: 2011-10-05
HENAN UNIV OF SCI & TECH
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AI Technical Summary

Problems solved by technology

[0005] Problems encountered in the production process of β-cyclodextrin: β-cyclodextrin produc

Method used

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Example Embodiment

[0025] Example 1

[0026] The following is a specific embodiment of the method for producing saccharification enzyme using β-cyclodextrin fermentation waste liquid of the present invention.

[0027] 1. Preparation of liquid seeds

[0028] Under sterile conditions, use a sterile pipette to aspirate 0.1 ml of thawed bacterial balls containing 10 3 The spore-free Aspergillus niger liquid per mL was spread on starch plate medium and incubated at 34°C for 5 days; then 1-2 colonies with regular shape and internal depressions were picked on the starch medium and placed on the test plate. The medium was streaked and separated at 34°C for 5 days. After maturity, it was placed in a refrigerator at 0-4°C for storage; the semi-dry medium was prepared, and after sterilization, the inoculation shovel or ring was used to take the test. A single mature colony on the Petri dish medium was placed in the sterilized semi-dried Chardonnay, with a liquid volume of 2 / 5, and incubated at a constant...

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Abstract

The invention provides a method for producing saccharifying enzyme by using beta-cyclodextrin fermentation waste liquor, In the method, aspergillus niger fermentation liquor is prepared by taking a liquid strain containing asporous aspergillus niger as a strain; and the method comprises the following steps of: inoculating the liquid strain in an amount which is 15 percent of the liquid volume into an aspergillus niger culture medium for fermentation, and performing gradual amplification culture by using a culture medium containing the beta-cyclodextrin fermentation waste liquor under certain fermentation conditions to obtain the aspergillus niger fermentation liquor which can directly serve as a saccharifying enzyme product. The beta-cyclodextrin fermentation waste liquor partially replaces starch for production, the production cost is saved and enterprise profit is increased; and the waste liquor is reutilized, the waste is changed into treasure, the treatment of the waste liquor is avoided and the environmental protection pressure is lightened.

Description

[0001] technical field [0002] The invention relates to a biological fermentation technology, in particular to a method for producing glucoamylase by using β-cyclodextrin fermentation waste liquid. Background technique [0003] Glucoamylase (abbreviated GA or G) is the abbreviation of Glucoamylase (Glucoamylase EC 3.2.1.3), also known as γ-amylase, is one of the most important industrial enzyme preparations, it is an exoglycosidase, from The non-reducing end of starch hydrolyzes α-1,4 glycosidic bonds in turn, cuts off each glucose unit, and, like β-amylase, changes the configuration of the hydrolyzed glucose to form β-D-glucose. As for amylopectin, when it encounters a branch point, it can also hydrolyze the α-1,6 glycosidic bond, thereby fully hydrolyzing the amylopectin to glucose. [0004] Glucoamylase is one of the most widely used enzymes in industry, and it is the most produced enzyme variety in China. The main use is as a starch saccharifying agent, so it is widel...

Claims

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Application Information

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IPC IPC(8): C12R1/685C12N9/34
Inventor 赵启美何佳孙晓菲张宏森张占洋李爱江关随霞
Owner HENAN UNIV OF SCI & TECH
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