Method for detecting FMDV (Foot and Mouth Disease Virus) through real-time fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction)

Abstract

The invention discloses a method for detecting an FMDV (Foot and Mouth Disease Virus) group and O type and AsiaI type thereof through real-time fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction). By carrying out specificity and sensitivity verification on the method established by the invention, the result shows that the FMDV can be well set apart from other Artiodactyla infectious viruses as well as O type and AsiaI type, and the sensitivity can be up to 10 copies of a template. The method established by the invention is characterized in that the whole reaction process is a closed pipe operation, thereby maximally reducing the pollution to the system. The whole process sequentially including the acquisition of a sample to be detected, the extraction of nucleic acid, the preparation of a fluorescent quantitative RT-PCR system and the representation of a reaction result can be completed within 2.5 hours, and the time required for detection is greatly shortened on the premise of ensuring the detection result.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method capable of accurately, specifically and rapidly detecting foot-and-mouth disease virus group specificity, type O specificity and AsiaI specificity. Background technique [0002] Foot and mouth disease (FMD) is an acute, febrile and contact infectious disease caused by cloven-hoofed animals caused by FMDV (Foot and mouth disease virus) infection. The World Organization for Animal Health (OIE) classifies the disease as a Category A disease due to its rapid onset and high contagiousness, which can have severe impacts. Once the disease is detected, the transportation and export of corresponding livestock and livestock products will be prohibited, causing huge economic and political impact. In 2005, my country announced the outbreak of Asia I type FMD. In addition to the previous reports of FMD, there have been two serotypes of FMD in China. The outbreak and prevalence of...

AI Technical Summary

Problems solved by technology

These methods are cumbersome and time-consuming to operate, and their specificity and sensitivity cannot meet the current detection needs.

In the virus isolation test, the process of culturing cells is particularly careful to control contamination, and the pathogenicity of virus materials and their adaptability to cells are different. Bacteria and mycoplasma can also pollute cells, so the experimental results obtained are often different.

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