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Immunofluorecence technique based method for evaluating activities of cryptosporidium parvum and giardia

A cryptosporidium and evaluation method technology, applied in biological testing, material inspection products, etc., can solve the problems of large errors in the evaluation of the effect of UV inactivation of Cryptosporidium and Giardia, high professional operation level requirements, and high cost. , to achieve the effect of low professional operation requirements, easy promotion and application, and high sensitivity

Inactive Publication Date: 2011-11-23
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of large error in the evaluation of UV inactivation of Cryptosporidium and Giardia, the cost is high, and the requirements for professional operation level are relatively high, the present invention proposes a new method for evaluating the activity of Cryptosporidium and Giardia

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  • Immunofluorecence technique based method for evaluating activities of cryptosporidium parvum and giardia
  • Immunofluorecence technique based method for evaluating activities of cryptosporidium parvum and giardia
  • Immunofluorecence technique based method for evaluating activities of cryptosporidium parvum and giardia

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example 1

[0037] The method disclosed in this example is to specifically analyze the method for evaluating the activity of two insects by immunofluorescence technology by collecting raw water that has not been subjected to ultraviolet light disinfection treatment as a water sample. Therefore, this example includes a UV disinfection step from the raw water followed by an evaluation of the activity of the two insects. The method for pretreatment of water samples after ultraviolet disinfection treatment disclosed in the above specific embodiments is also applicable to the pretreatment of raw water, and can achieve the same evaluation results.

[0038] Apply the above method for evaluating the activity of Cryptosporidium oocysts and Giardia cysts by UV irradiation, and the specific steps are as follows:

[0039] (1) Provide the water to be treated, and carry out the purification and collection of the two worms: After the water samples to be treated are collected, they are filtered through 6...

example 2

[0048] According to the method described in Example 1, set different light doses Light dosage (mJ / cm 2 ) and light intensity (mW / cm 2 ), irradiate the experimental samples, and evaluate the inactivation rate of the two worms by fluorescent activity staining method (DAPI / PI). Other steps and parameters are the same as Example 1. The irradiation light intensity is respectively: 1.1, 2.3, 4.8mW / cm 2 . Experimental results refer to figure 2 , this figure shows that after different irradiation times, the irradiation light dose Light dosage (mJ / cm 2 ) on the inactivation race (%) of the inactivation rate of the two worms, and the detection took 5 hours. From figure 2 It can be seen from the figure that the UV dose must be greater than 460 mJ / cm when the inactivation rate is 99.9% (3-log) using the fluorescence activity staining method (DAPI / PI). 2 .

example 3

[0050] According to the method described in Example 1, set different light doses Light dosage (mJ / cm 2 ) and light intensity (mW / cm 2 ), irradiate the experimental sample, and evaluate the inactivation rate of the two worms according to the method of Example 1. Other steps and parameters are the same as Example 1. The irradiation light intensity is respectively: 1.1, 2.2, 4.5mW / cm 2 . Experimental results refer to image 3 , this figure shows that after different irradiation times, using the immunofluorescence evaluation method, the irradiation light dose Light dosage (mJ / cm 2 ) on the inactivation race (%) of the inactivation rate of the two worms, and the detection took 6 hours. From image 3 It can be seen from the figure that using the immunological TD (pyrimidine dimer) fluorescence method UV dosage=22 mJ / cm 2 (1.1mW / cm 2 ) can reach 3-log (99.9%) inactivation rate. Explanation: The immunological TD (pyrimidine dimer) fluorescence method has obvious advantages in...

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Abstract

The invention provides an immunofluorecence technique based method for evaluating activities of cryptosporidium parvum and giardia. The method has the following beneficial effects: the accurate, quick, simple, convenient and low-cost high throughput immunofluorecence assay technique is established and is used for evaluating the activities of cryptosporidium parvum and giardia in the ultraviolet (UV) disinfectant fluid; evaluation of the activities of cryptosporidium parvum and giardia in the UV irradiated water is realized by mainly carrying out competitive immunobinding on anti-pyrimidine-dimer (TDs) monoclonal antibodies and the TDs, taking fluorescein isothiocyanate as a marker and immunoglobulin (IgG) as a fluorescent substrate and binding 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI); compared with the fluorescent staining method for evaluating the effect of UV on inactivating cryptosporidium parvum and giardia, the method provided by the invention has such excellent characteristics as strong specificity, high sensitivity and low professional operation requirements; and the cryptosporidium parvum and giardia activity evaluation method system is perfected by binding the method provided by the invention with the fluorescent staining method and other methods, and the method provided by the invention can be widely applied to the fields such as water quality monitoring, disease control and epidemic prevention and the like.

Description

technical field [0001] The present invention relates to Cryptosporidium ( Cryptosporidium parvum ) and Giardia ( Giardia ) activity evaluation method, especially related to the cryptosporidium and giardia activity evaluation method based on immunofluorescence technology. Background technique [0002] The pollution of drinking water by Cryptosporidium and Giardia has posed a serious threat to the health of citizens, and Cryptosporidium oocysts and Giardia cysts (hereinafter referred to as "two worms") have become media of concern. One of the risk factors for waterborne organisms. In my country's newly promulgated "Drinking Water Sanitation Standards", Cryptosporidium and Giardia have been used as unconventional indicators and their limit values ​​have been specified. From the point of view, there is a potential risk of "two worms" outbreak. In order to ensure the safety of drinking water, it is of great and urgent practical significance to develop an efficient and low-cost...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 李绍峰冉治霖朱静赖春娜杨昕
Owner SHENZHEN POLYTECHNIC
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